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タイトル :High performance thin-layer chromatography and image processing analysis of some essential oils from Perovskia atriplicifolia leaves extracted by microwave assisted heating
著者 :FAQERYAR, Nazifa
MORI, Yoshihito
発行日 :2016-11
抄録 :A high performance thin layer chromatography (HPTLC) with support of an image processing method has been developed and validated for qualitative and quantitative analysis of β-caryophyllene and d-camphor from Perovskia atriplicifolia essential oil extracted by microwave assisted heating and hydrodistillation methods. The HPTLC analysis was performed in a twin trough chamber on silica gel 60 sheet using toluene-ethyl acetate (95:5, v: v) as mobile phase and anisaldehyde solution as derivatizing reagent. The scanned digital images of sheets were analyzed by means of an ImageJ software. The Rf values examined by ImageJ for β-caryophyllene and d-camphor were 0.20±0.05 and 0.85±0.30, respectively. A linear relationship was found in the range of 0.905-5.43 μg/spot for β-caryophyllene and 0.025-0.15 mg/spot for d-camphor. The quantification for both analyts were found to be 1.79 μg/spot and 0.13 mg/spot, and detection limit 0.53 μg/spot and 0.04 mg/spot, respectively. The results obtained in validation represent good accuracy and precision of the developed HPTLC methods.
資料種別 :紀要論文
URI :http://hdl.handle.net/10083/60706
NII書誌ID :AN00033958
公開者・出版者 :お茶の水女子大学
掲載雑誌 :お茶の水女子大學自然科學報告 Vol.67 no.1 p.1 -11
出現コレクション:お茶の水女子大學自然科學報告