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Binding of sulfatide to concanavalin A and Lens culinaris agglutinin
http://hdl.handle.net/10083/35523
http://hdl.handle.net/10083/35523f0e6fdc7-4fc7-4448-b2d5-66a4e1cbdf4d
名前 / ファイル | ライセンス | アクション |
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03_027-035.pdf (701.7 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2010-01-28 | |||||
タイトル | ||||||
タイトル | Binding of sulfatide to concanavalin A and Lens culinaris agglutinin | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | lectin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | sulfatide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | concanavalin A (Con A) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lens culinaris agglutinin (LCA) | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
著者 |
Yousofzai, Wahida
× Yousofzai, Wahida× Kashimoto, Miwa× Tanaka, Hideko× Matsumoto, Isamu× Kojima-Aikawa, Kyoko× Kobayashi, Tetsuyuki |
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著者(ヨミ) | ||||||
識別子 | 71006 | |||||
識別子Scheme | WEKO | |||||
姓名 | かしもと, みわ | |||||
著者(ヨミ) | ||||||
識別子 | 71007 | |||||
識別子Scheme | WEKO | |||||
姓名 | たなか, ひでこ | |||||
著者(ヨミ) | ||||||
識別子 | 71008 | |||||
識別子Scheme | WEKO | |||||
姓名 | まつもと, いさむ | |||||
著者(ヨミ) | ||||||
識別子 | 71009 | |||||
識別子Scheme | WEKO | |||||
姓名 | こじま-あいかわ, きょうこ | |||||
著者(ヨミ) | ||||||
識別子 | 71010 | |||||
識別子Scheme | WEKO | |||||
姓名 | こばやし, てつゆき | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Lectins, carbohydrate binding proteins of non-immune origin, are characterized by a high degree of specificity in binding to some saccharides in preference to others. Some lectins are reported to interact with sulfated glycosaminoglycans, but little is known about an interaction between lectins and sulfatide. Here, we examined the binding of sulfatide to leguminous plant lectins, such as Lens culinaris agglutinin (LCA), concanavalin A (Con A), peanut agglutinin, soybean agglutinin, Solanum tuberosum agglutinin, Ulex europaeus agglutinin, and Bauhinia purpurea agglutinin. A solid phase binding assay using horse radish peroxidase-labeled lectins showed that LCA and Con A could bind to sulfatide coated on microtiter plates, but failed to bind to galactosylceramide. The binding of these lectins to sulfatide was inhibited in the presence of excess amounts of either methyl α-D-mannopyranoside or D-glucose, whereas it was not affected by D-galactose. Furthermore, both Con A and LCA bound to sulfatide which was incorporated into lipid vesicles and the binding was inhibited by addition of methyl α-D-mannopyranoside. These\ results suggest that Con A and LCA have a binding site of sulfatide whose affinity is affected by the specific binding of saccharides. |
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書誌情報 |
お茶の水女子大學自然科學報告 巻 59, 号 2, p. 27-35, 発行日 2009-02-27 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00298190 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00033958 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
形態 | ||||||
701695 bytes | ||||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 400 | |||||
出版者 | ||||||
出版者 | お茶の水女子大学 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | 紀要論文 | |||||
資源タイプ・ローカル | ||||||
紀要論文 | ||||||
資源タイプ・NII | ||||||
Departmental Bulletin Paper | ||||||
資源タイプ・DCMI | ||||||
text | ||||||
資源タイプ・ローカル表示コード | ||||||
03 | ||||||
所属 | ||||||
Department of Biological Sciences, Graduate School of Humanities and Sciences, Ochanomizu University | ||||||
所属 | ||||||
Department of Biological Sciences, Graduate School of Humanities and Sciences, Ochanomizu University | ||||||
所属 | ||||||
Department of Biological Sciences, Graduate School of Humanities and Sciences, Ochanomizu University | ||||||
所属 | ||||||
Department of Biological Sciences, Graduate School of Humanities and Sciences, Ochanomizu University | ||||||
所属 | ||||||
Department of Biological Sciences, Graduate School of Humanities and Sciences, Ochanomizu University | ||||||
所属 | ||||||
Department of Biological Sciences, Graduate School of Humanities and Sciences, Ochanomizu University |