WEKO3
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Characterization of Nuclear, and Nuclear Matrix-Bound DNA Polymerases of Normal Rat Liver
http://hdl.handle.net/10083/838
http://hdl.handle.net/10083/8380757497c-09fb-4cd4-a240-6e16e5a9efcf
名前 / ファイル | ライセンス | アクション |
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KJ00004470824.pdf (321.6 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2007-04-23 | |||||
タイトル | ||||||
タイトル | Characterization of Nuclear, and Nuclear Matrix-Bound DNA Polymerases of Normal Rat Liver | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | nuclear matrix | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA synthesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | rat liver | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
著者 |
Uchiyama, Nahoko
× Uchiyama, Nahoko× Kohyama, Noriko× Shimada, Yukiko× Murakami-Murofushi, Kimiko |
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著者(ヨミ) | ||||||
識別子 | 70563 | |||||
識別子Scheme | WEKO | |||||
姓名 | ウチヤマ, ナホコ | |||||
著者(ヨミ) | ||||||
識別子 | 70564 | |||||
識別子Scheme | WEKO | |||||
姓名 | コウヤマ, ノリコ | |||||
著者(ヨミ) | ||||||
識別子 | 70565 | |||||
識別子Scheme | WEKO | |||||
姓名 | シマダ, ユキコ | |||||
著者(ヨミ) | ||||||
識別子 | 70566 | |||||
識別子Scheme | WEKO | |||||
姓名 | ムラカミ-ムロフシ, キミコ | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | DNA polymerases in the nuclei and those bound to the nuclear matrices isolated from normal rat liver (at G_0 phase) were characterized. The nuclei from 1 gram tissue contained 0.46 unit of DNA polymerase α (0.05U/mg protein) and 19 units of DNA polymerase β (2.2U/mg), but the nuclear matrices from 1 gram tissue had less than 0.01 unit of DNA polymerase α (2% of the activity in the nuclei) and 0.4 unit of DNA polymerase β (2% of the nuclear activity). In contrast, regenerating rat liver (at S-phase) contained more than ten times higher activity of DNA polymerase α in the nuclei (5.2U/g tissue) and 20% of the activity of this enzyme was bound to nuclear matrices. S-phase nuclei contained almost the same activity of DNA polymerase β (21 units/g tissue) as that in the G_0 nuclei, and 0.5% of the β activity was bound to the nuclear matrices. These findings indicate that no functional replication complex is assembled in the G_0 nuclei and no significant DNA polymerases are bound to the nuclear matrices. | |||||
書誌情報 |
お茶の水女子大學自然科學報告 巻 45, 号 1, p. 79-83, 発行日 1994-07-15 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00298190 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00033958 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
形態 | ||||||
321551 bytes | ||||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 400 | |||||
出版者 | ||||||
出版者 | お茶の水女子大学 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | 紀要論文 | |||||
資源タイプ・ローカル | ||||||
紀要論文 | ||||||
資源タイプ・NII | ||||||
Departmental Bulletin Paper | ||||||
資源タイプ・DCMI | ||||||
text | ||||||
資源タイプ・ローカル表示コード | ||||||
03 | ||||||
所属 | ||||||
Department of Biology, Faculty of Science, Ochanomizu University | ||||||
所属 | ||||||
Department of Biology, Faculty of Science, Ochanomizu University | ||||||
所属 | ||||||
Department of Biology, Faculty of Science, Ochanomizu University | ||||||
所属 | ||||||
Department of Biology, Faculty of Science, Ochanomizu University |