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Pseudoproteoglycan (pseudoPG) probes that simulate PG macromolecular structure for screening and isolation of PG-binding proteins
http://hdl.handle.net/10083/35135
http://hdl.handle.net/10083/35135b2395de2-9a0e-492a-befc-0b55f850667a
名前 / ファイル | ライセンス | アクション |
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Glycoconjugate Journal 2009 Feb 21. [Epub ahead of print].doc.pdf (782.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-01-26 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Pseudoproteoglycan (pseudoPG) probes that simulate PG macromolecular structure for screening and isolation of PG-binding proteins | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Glycosaminoglycan | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Heparin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | binding proteins | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cyclophilin A | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HSP90 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Amphoterin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Detection and separation | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
Nakagawa, Keiko
× Nakagawa, Keiko× Nakamura, Kosuke× Haishima, Yuji× Yamagami, Makiko× Saito, Kana× Sakagami, Hiromi× Ogawa, Haruko |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A proteoglycan (PG) monomer is a macromolecule consisting of one or more glycosaminoglycan (GAG) chains attached to a core protein. PGs have signaling roles and modulatory functions in the extracellular matrix and at the cell surface. To elucidate the functions of higher-order PG structures, pseudoPGs that imitate the PG structure were prepared to develop probes and affinity adsorbents. Poly-L-lysine (PLL) or polyacrylamide (PAA) was coupled with various GAGs, then biotinylated, and the remaining amino groups were blocked to obtain the pseudoPG probes, biotinyl PLL (BPL)- or PAA (BPA)-GAGs. Lactoferrin exhibited 30-times higher affinity toward BPL-heparin than the conventional single-strand probe, biotin-hydrazide-heparin.Heparin-PLL was immobilized on a formyl-Sepharose and compared with the Hep-Sepharose in which heparin was directly immobilized to amino-Sepharose. Screening for ligands in normal rat brain revealed several proteins that specifically bound to either of the two adsorbents, indicating that the heparin-binding proteins exhibit specific recognition depending on the higher-order structure of the PG. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | online first / <br/>Received: 28 July 2008 / Revised: 30 September 2008 / Accepted: 8 December 2008 / Published online: 21 February 2009 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The original publication is available at www.springerlink.com | |||||
書誌情報 |
Glycoconjugate Journal 発行日 2009-02-21 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 02820080 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11981860 | |||||
権利 | ||||||
権利情報 | Springer Science + Business Media, LLC 2009 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | text/plain | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
形態 | ||||||
782111 bytes | ||||||
形態 | ||||||
41649 bytes | ||||||
形態 | ||||||
27311 bytes | ||||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
著者版フラグ | ||||||
値 | author | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 464 | |||||
NIIサブジェクト | ||||||
主題Scheme | Other | |||||
主題 | 生物学 | |||||
出版者 | ||||||
出版者 | Springer Netherlands | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | 学術雑誌論文 | |||||
資源タイプ・ローカル | ||||||
学術雑誌論文 | ||||||
資源タイプ・NII | ||||||
Journal Article | ||||||
資源タイプ・DCMI | ||||||
text | ||||||
資源タイプ・ローカル表示コード | ||||||
01 | ||||||
異版である | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | https://doi.org/10.1007/s10719-008-9220-y | |||||
参照する | ||||||
関連タイプ | references | |||||
識別子タイプ | URI | |||||
関連識別子 | http://www.springerlink.com/content/880502ul280187v5/ |