A lectin highly reactive with dermatan sulfate (DS-lectin) was purified from adult chicken liver using new affinity adsorbents which were prepared by immobilizing dermatan sulfate or heparin at the reducing ends on hydrazino-Toyopearl. The DS-lectin behaved as a single protein with a molecular weight of approx. 180,000 by gel chromatography, but could be further dissociated into three proteins with apparent molecular weights of 40,000, 47,000 and 87,000 on SDS polyacrylamide gel electrophoresis. On excitation at 280nm, the DS-lectin emitted fluorescence centered at 336nm, which was attributable to tryptophan residues and could be quenched by the addition of specific saccharides. The association constants of the DS-lectin with specific saccharides were calculated from changes in intensities of fluorescence-difference spectra induced by the saccharides. Dermatan sulfate and protuberic acid, which is composed of L-iduronic acid and D-glucuronic acid (1:2), had the highest association constants among polysaccharides tested. Partially N-desulfated heparin had a higher association constant than that of native heparin whi\
le dextran sulfate had no affinity. D-Glucuronic acid and N-acetylneuraminic acid induced weak but significant quenching, but not N-acetylgalactosamine or cellobiose. These results were essentially in agreement with those of hemagglutination inhibition tests and indicated that DS-lectin has a strong affinity for L-iduronic acid residues and probably carboxyl groups in the saccharides, while sulfate groups on the saccharides interfere with the specific interaction.
雑誌名
お茶の水女子大學自然科學報告
巻
36
号
1
ページ
89 - 102
発行年
1985-07
ISSN
00298190
書誌レコードID
AN00033958
フォーマット
application/pdf
形態
1122585 bytes
日本十進分類法
400
出版者
お茶の水女子大学
資源タイプ
紀要論文
資源タイプ・ローカル
紀要論文
資源タイプ・NII
Departmental Bulletin Paper
資源タイプ・DCMI
text
資源タイプ・ローカル表示コード
03
所属
Department of Chemistry, Faculty of Science, Ochanomizu University
Department of Chemistry, Faculty of Science, Ochanomizu University
Department of Chemistry, Faculty of Science, Ochanomizu University
Department of Chemistry, Faculty of Science, Ochanomizu University
Studies on Chicken Liver Lectin
KJ00004830608.pdf
(1.12MB)
