{"created":"2021-03-01T06:39:56.626291+00:00","id":34641,"links":{},"metadata":{"_buckets":{"deposit":"7bbf8892-a306-4fd9-a647-655877423eba"},"_deposit":{"id":"34641","owners":[],"pid":{"revision_id":0,"type":"depid","value":"34641"},"status":"published"},"_oai":{"id":"oai:teapot.lib.ocha.ac.jp:00034641","sets":["347:359:680"]},"item_11_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"58","bibliographicPageStart":"41","bibliographicVolumeNumber":"55","bibliographic_titles":[{"bibliographic_title":"お茶の水女子大學自然科學報告"}]}]},"item_11_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_11_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"紀要論文","subitem_description_type":"Other"}]},"item_11_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"We previously demonstrated that annexins are localized in exocrine tissues such as kidney, liver, intestine and pancreatic acinar cells. We also elucidated the glycosaminoglycan (GAG) binding properties of annexins IV, V and VI and showed that each of them has its own specific binding activities to GAGS. In this study, we examined the function of annexin VI and GAGs in pancreatic β cells. Immunohistochemical studies and immunoblot assays showed that annexin VI localized in rat pancreatic islets, HIT-T15 cells derived from rat insulinorna and MIN6 cells derived from mouse insulinoma. Immunoblot assay detected heparan sulfate proteoglycan in those cell lysates but not chondroitin sulfate proteoglycan. Affinity adsorption assay showed annexin VI bound to the heparan sulfate proteoglycans derived from HIT-T15 cells indicating that these proteoglycans the endogenous ligand of annexin VI in β cells. Insulin secretion assay from permeabilized MIN6 cells showed that full-length human annexin VI promotes insulin release, but not other mutants. Inhibition of Ca^<2+>-dependent insulin release by GAGs was inferred that GAGs bl\\\nocked binding of annexin VI and membrane vesicle. These results suggest that annexin VI was considered to exit in pancreatic endocrine cells, and to interact with both cell-extracellular matrix heparan sulfate proteoglycan and membrane vesicle in β cells.","subitem_description_type":"Other"}]},"item_11_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"70872","nameIdentifierScheme":"WEKO"}],"names":[{"name":"シムラ, ユミコ"}]},{"nameIdentifiers":[{"nameIdentifier":"70873","nameIdentifierScheme":"WEKO"}],"names":[{"name":"イダ, ミチル"}]},{"nameIdentifiers":[{"nameIdentifier":"70874","nameIdentifierScheme":"WEKO"}],"names":[{"name":"キマタ, コウジ"}]},{"nameIdentifiers":[{"nameIdentifier":"70875","nameIdentifierScheme":"WEKO"}],"names":[{"name":"マツモト, イサム"}]}]},"item_11_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"お茶の水女子大学"}]},"item_11_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00033958","subitem_source_identifier_type":"NCID"}]},"item_11_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00298190","subitem_source_identifier_type":"ISSN"}]},"item_11_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"400","subitem_subject_scheme":"NDC"}]},"item_11_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"1314615 bytes"}]},"item_11_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"紀要論文"}]},"item_11_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper"}]},"item_11_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_11_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"03"}]},"item_11_text_79":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_text_value":"Graduate School Of Humanities and Sciences"},{"subitem_text_value":"Graduate School Of Humanities and Sciences"},{"subitem_text_value":"Institute for Molecular Science of Medicine, Aichi Medical University"},{"subitem_text_value":"Graduate School Of Humanities and Sciences:Department of Chemistry, Faculty of Science, Ochanomizu University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shimura, Yumiko Eris"}],"nameIdentifiers":[{"nameIdentifier":"70868","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ida, Michiru"}],"nameIdentifiers":[{"nameIdentifier":"70869","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kimata, Koji"}],"nameIdentifiers":[{"nameIdentifier":"70870","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumoto, Isamu"}],"nameIdentifiers":[{"nameIdentifier":"70871","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-19"}],"displaytype":"detail","filename":"KJ00004830782.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004830782.pdf","url":"https://teapot.lib.ocha.ac.jp/record/34641/files/KJ00004830782.pdf"},"version_id":"9be27123-977b-4683-9f5b-05a219a2cc7c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Annexins and Heparan Sulfate Proteoglycans in Pancreatic Endocrine β Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Annexins and Heparan Sulfate Proteoglycans in Pancreatic Endocrine β Cells"}]},"item_type_id":"11","owner":"1","path":["680"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-04-30"},"publish_date":"2008-04-30","publish_status":"0","recid":"34641","relation_version_is_last":true,"title":["Annexins and Heparan Sulfate Proteoglycans in Pancreatic Endocrine β Cells"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-12T05:39:03.225945+00:00"}