@article{oai:teapot.lib.ocha.ac.jp:00034653,
 author = {Ueda, Haruko and Hatanaka, Yasumaru and Ogawa, Haruko},
 issue = {2},
 journal = {お茶の水女子大學自然科學報告},
 month = {Dec},
 note = {application/pdf, 紀要論文, Mannose/glucose-specific sophoragrin, a Sophora japonica bark lectin, shows unique multimerization and dissociation accompanied by transitions between insolubility and solubility, which are modulated by both a specific sugar and Ca^<2+> concentration. Sophoragrin is composed of four subunits, a-1, a-2, b-1, and b-2, and all except for b-2 are reported to be glycosylated. Biotinylated sophoragrin bound sugar-specifically to subunit 'b' but not to subunit 'a' on the membrane. Sophoragrin was labeled by only one of the photoaffinity-probes derived from sophoragrin-glycans, and the labeling was inhibited by a specific sugar. In contrast, another probe-mixture, which contains major oligosaccharide of sophoragrin (Man_3Xyl_1Fuc_1GlcNAc_2), did not label sophoragrin. These results reveal that the multimerization of sophoragrin is caused by a particular signal oligosaccharide on subunit 'b'. It was shown that sophoragrin can form a soluble complex with other endogenous glycoproteins, and may play a specific role through its transition between soluble and insoluble forms.},
 pages = {45--52},
 title = {Leguminous wood has a unique vacuolar lectin in bark : Lectin-carbohydrate interaction modulates solubility-insolubility transition of vacuolar glycoproteins},
 volume = {53},
 year = {2002}
}