{"created":"2021-03-01T06:39:59.936893+00:00","id":34689,"links":{},"metadata":{"_buckets":{"deposit":"67fe0b1c-8397-4a37-9933-b7943095cc7a"},"_deposit":{"id":"34689","owners":[],"pid":{"revision_id":0,"type":"depid","value":"34689"},"status":"published"},"_oai":{"id":"oai:teapot.lib.ocha.ac.jp:00034689","sets":["347:359:670"]},"item_11_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-07-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"45","bibliographicPageStart":"31","bibliographicVolumeNumber":"50","bibliographic_titles":[{"bibliographic_title":"お茶の水女子大學自然科學報告"}]}]},"item_11_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_11_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"紀要論文","subitem_description_type":"Other"}]},"item_11_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"A lectin from the fruiting body of Psathyrella velutina (PVL) has been reported to be specific for nonreducing terminal N-acetylglucosamine (GlcNAc) residue. PVL was found to exhibit multispecificity toward acidic glycoconjugates, i.e., polysaccharides, sialoglycoproteins, and sulfatide. PVL was purified by single-step affinity chromatography on an N-acetylchitooligosaccharides (GlcNAc_<5-6>)-Sepharose column. The binding specificities of PVL were studied using neoproteoglycans and neoglycoproteins involving heparin or GlcNAc_<5-6>. PVL was shown, by both membrane analysis and solid phase assay, to bind with heparin neoproteoglycans. The binding was inhibited by polygalacturonic acid, dextran sulfate, or fucoidan as well as heparin, but not by GlcNAc, other glycosaminoglycans, colominic acid, or DNA. The pH-dependencies of the binding to heparin and to GlcNAc_<5-6> were different. Biotinylation of PVL destroyed the heparin binding activity while retaining the GlcNAc binding activity. In addition, circular dichroism spectroscopy of PVL indicated that a small conformational change of PVL is induced by binding with GlcN\\\nAc but not with heparin, suggesting that the binding sites for acidic polysaccharides and GlcNAc are independently located on PVL. On the other hand. PVL bound to sialoglycoproteins on solid phase assays, and the binding was inhibited with GlcNAc or desialylation treatment, indicating that PVL recognizes N-acetylneuraminic acid residue in glycoproteins at GlcNAc-binding site. The binding of PVL to sulfatide was not inhibited by GlcNAc, N-acetylneuraminic acid, or heparin. These findings indicate that the binding specificities of PVL to glycoconjugates are quite different from those reported for oligosaccharides and PVL is a novel member of muluspecific lectin in higher fungi.","subitem_description_type":"Other"}]},"item_11_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"70663","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ウエダ, ハルコ"}]},{"nameIdentifiers":[{"nameIdentifier":"70664","nameIdentifierScheme":"WEKO"}],"names":[{"name":"コジマ, キョウコ"}]},{"nameIdentifiers":[{"nameIdentifier":"70665","nameIdentifierScheme":"WEKO"}],"names":[{"name":"サイトウ, タケシ"}]},{"nameIdentifiers":[{"nameIdentifier":"70666","nameIdentifierScheme":"WEKO"}],"names":[{"name":"オガワ, ハルコ"}]}]},"item_11_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"お茶の水女子大学"}]},"item_11_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00033958","subitem_source_identifier_type":"NCID"}]},"item_11_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00298190","subitem_source_identifier_type":"ISSN"}]},"item_11_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"400","subitem_subject_scheme":"NDC"}]},"item_11_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"1178164 bytes"}]},"item_11_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"紀要論文"}]},"item_11_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper"}]},"item_11_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_11_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"03"}]},"item_11_text_79":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_text_value":"Department of Advanced Biosciences, Graduate School of Humanities and Sciences, Ochanomizu University"},{"subitem_text_value":"Department of Chemistry, Faculty of Science, Ochanomizu University"},{"subitem_text_value":"The Mushroom Research Institute of Japan"},{"subitem_text_value":"Department of Advanced Biosciences, Graduate School of Humanities and Sciences, Ochanomizu University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ueda, Haruko"}],"nameIdentifiers":[{"nameIdentifier":"70659","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kojima, Kyoko"}],"nameIdentifiers":[{"nameIdentifier":"70660","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saitoh, Takeshi"}],"nameIdentifiers":[{"nameIdentifier":"70661","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ogawa, Haruko"}],"nameIdentifiers":[{"nameIdentifier":"70662","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-19"}],"displaytype":"detail","filename":"KJ00004470905.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004470905.pdf","url":"https://teapot.lib.ocha.ac.jp/record/34689/files/KJ00004470905.pdf"},"version_id":"0f18ca39-e16c-4267-8135-1f510fda3a38"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Multi-specificities of the N-Acetylglucosamine-Binding Lectin from Psathyrella velutina Mushroom toward Acidic Glycoconjugates","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Multi-specificities of the N-Acetylglucosamine-Binding Lectin from Psathyrella velutina Mushroom toward Acidic Glycoconjugates"}]},"item_type_id":"11","owner":"1","path":["670"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-04-23"},"publish_date":"2007-04-23","publish_status":"0","recid":"34689","relation_version_is_last":true,"title":["Multi-specificities of the N-Acetylglucosamine-Binding Lectin from Psathyrella velutina Mushroom toward Acidic Glycoconjugates"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-12T06:09:46.051364+00:00"}