{"created":"2021-03-01T06:40:00.847368+00:00","id":34702,"links":{},"metadata":{"_buckets":{"deposit":"db48099c-fa5a-4fea-8251-6fc01027b3bf"},"_deposit":{"id":"34702","owners":[],"pid":{"revision_id":0,"type":"depid","value":"34702"},"status":"published"},"_oai":{"id":"oai:teapot.lib.ocha.ac.jp:00034702","sets":["347:359:665"]},"item_11_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-12-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"34","bibliographicPageStart":"29","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"お茶の水女子大學自然科學報告"}]}]},"item_11_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_11_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"紀要論文","subitem_description_type":"Other"}]},"item_11_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Under high salt conditions, haploid myxoamoebae of a true slime mold, Physanun polycephalum retract their pseudopodia and change their cell shape into disk-like form, after which they construct the cell wall to form their dormant type designated microcysts. We observed the change of the intracellular distribution of actin filaments associated with the cellular morphological changes. Staining with phalloidin showed that the actin filaments were almost uniformly distributed throughout the myxoamoebae cytoplasm. When these cells were exposed to salt stress, the actin structures changed into short rods or dots, and they localized along the periphery of the cell. An incubation of the myxoamoebae in high salt medium, caused the synthesis of several species proteins, among which a 66-kD protein (p66) was most prominently induced.We found that p66 was co-precipitated with polymerized actin and bound to ATP-agarose. A double staining of the disk-shaped cells with anti-p66 antibody and phalloidin revealed superimposable localization of p66 and actin filaments in the short rods or dots. And it was revealed that p66 was immunolo\\\ngically unrelated to the common heat shock protein,HSP70 and HSP90,those are highly conserved during evolution. From these results, p66 is supposed to be a novel stress protein and interact with actin to perticipate in the morphological changes of the cell induced by salt stress.","subitem_description_type":"Other"}]},"item_11_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"70585","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ウチヤマ, ナオコ"}]},{"nameIdentifiers":[{"nameIdentifier":"70586","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ムラカミ-ムロフシ, キミコ"}]}]},"item_11_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"お茶の水女子大学"}]},"item_11_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00033958","subitem_source_identifier_type":"NCID"}]},"item_11_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00298190","subitem_source_identifier_type":"ISSN"}]},"item_11_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"400","subitem_subject_scheme":"NDC"}]},"item_11_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"674723 bytes"}]},"item_11_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"紀要論文"}]},"item_11_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper"}]},"item_11_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_11_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"03"}]},"item_11_text_79":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_text_value":"Department of Biology, Faculty of Science, Ochanomizu University"},{"subitem_text_value":"Department of Biology, Faculty of Science, Ochanomizu University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Uchiyama, Naoko"}],"nameIdentifiers":[{"nameIdentifier":"70583","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Murakami-Murofushi, Kimiko"}],"nameIdentifiers":[{"nameIdentifier":"70584","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-19"}],"displaytype":"detail","filename":"KJ00004470860.pdf","filesize":[{"value":"674.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004470860.pdf","url":"https://teapot.lib.ocha.ac.jp/record/34702/files/KJ00004470860.pdf"},"version_id":"ce913e8a-7e96-4aeb-95fc-ad460a0845dd"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"RESPONSE OF A TRUE SLIME MOLD, Physarum polycephalum, TO SALT STRESS","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"RESPONSE OF A TRUE SLIME MOLD, Physarum polycephalum, TO SALT STRESS"}]},"item_type_id":"11","owner":"1","path":["665"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-04-23"},"publish_date":"2007-04-23","publish_status":"0","recid":"34702","relation_version_is_last":true,"title":["RESPONSE OF A TRUE SLIME MOLD, Physarum polycephalum, TO SALT STRESS"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-12T05:39:10.591948+00:00"}