{"created":"2021-03-01T06:40:01.907097+00:00","id":34714,"links":{},"metadata":{"_buckets":{"deposit":"f964b768-e30f-4a82-8b98-e8646a59269b"},"_deposit":{"id":"34714","owners":[],"pid":{"revision_id":0,"type":"depid","value":"34714"},"status":"published"},"_oai":{"id":"oai:teapot.lib.ocha.ac.jp:00034714","sets":["347:359:660"]},"item_11_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-07-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"78","bibliographicPageStart":"67","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"お茶の水女子大學自然科學報告"}]}]},"item_11_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_11_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"紀要論文","subitem_description_type":"Other"}]},"item_11_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"In order to compare the characteristics of acid protease (s) in diploid and haploid cells of a true slime mold, Physarum polycephalum, an acid protease was purified from the haploid myxoamoebae of Physarum by a combination of detergent extraction, acid precipitation, and ion-exchange column chromatographies. The purified enzyme having a molecular weight of 68,000 was composed of two polypeptide chains, 31-kDa heavy chain and 23-kDa light chain. These polypeptides were cross-linked by disulfide bond (s), and the heavy chain contained carbohydrate moiety composed of mannose, glucosamine, fucose and glucose. Optimum pH of the enzyme reaction was 1.7 toward hemoglobin as a substrate. A typical aspartic protease inhibitor, diazoacetyl-D, L-norleucine methyl ester (DAN), inhibited the enzyme activity in the presence of cupric ions, but the enzyme was not sensitive to the other aspartic protease inhibitors, 1, 2-epoxy-3-(p-nitrophenoxy) propane (EPNP) and pepstatin A. These results indicate that the characteristics of the amoeboid protease is very similar to those of the plasmodial enzyme. In addition, amino acid compositio\\\nn and specific activity of the purified enzyme were also very similar to those of the plasmodial protease.","subitem_description_type":"Other"}]},"item_11_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"70556","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ミノワ-ソノベ, ユキエ"}]},{"nameIdentifiers":[{"nameIdentifier":"70557","nameIdentifierScheme":"WEKO"}],"names":[{"name":"シマダ, ユキコ"}]},{"nameIdentifiers":[{"nameIdentifier":"70558","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ムラカミ-ムロフシ, キミコ"}]}]},"item_11_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"お茶の水女子大学"}]},"item_11_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00033958","subitem_source_identifier_type":"NCID"}]},"item_11_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00298190","subitem_source_identifier_type":"ISSN"}]},"item_11_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"400","subitem_subject_scheme":"NDC"}]},"item_11_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"747381 bytes"}]},"item_11_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"紀要論文"}]},"item_11_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper"}]},"item_11_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_11_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"03"}]},"item_11_text_79":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_text_value":"Department of Biology, Faculty of Science, Ochanomizu University"},{"subitem_text_value":"Department of Biology, Faculty of Science, Ochanomizu University"},{"subitem_text_value":"Department of Biology, Faculty of Science, Ochanomizu University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Minowa-Sonobe, Yukie"}],"nameIdentifiers":[{"nameIdentifier":"70553","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimada, Ynkiko"}],"nameIdentifiers":[{"nameIdentifier":"70554","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Murakami-Murofushi, Kimiko"}],"nameIdentifiers":[{"nameIdentifier":"70555","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-19"}],"displaytype":"detail","filename":"KJ00004470823.pdf","filesize":[{"value":"747.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004470823.pdf","url":"https://teapot.lib.ocha.ac.jp/record/34714/files/KJ00004470823.pdf"},"version_id":"ea12bc26-0f4d-427f-93a3-a3b8c655e947"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Purification and Characterization of an Acid Protease from Myxoamoebae of a True Slime Mold, Physarum polycephalum","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Purification and Characterization of an Acid Protease from Myxoamoebae of a True Slime Mold, Physarum polycephalum"}]},"item_type_id":"11","owner":"1","path":["660"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-04-23"},"publish_date":"2007-04-23","publish_status":"0","recid":"34714","relation_version_is_last":true,"title":["Purification and Characterization of an Acid Protease from Myxoamoebae of a True Slime Mold, Physarum polycephalum"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-12T05:39:12.439748+00:00"}