{"created":"2021-03-01T06:40:02.902959+00:00","id":34728,"links":{},"metadata":{"_buckets":{"deposit":"f8046e4d-79c6-4503-909b-cac49b053547"},"_deposit":{"id":"34728","owners":[],"pid":{"revision_id":0,"type":"depid","value":"34728"},"status":"published"},"_oai":{"id":"oai:teapot.lib.ocha.ac.jp:00034728","sets":["347:359:657"]},"item_11_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1992-12-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"96","bibliographicPageStart":"75","bibliographicVolumeNumber":"43","bibliographic_titles":[{"bibliographic_title":"お茶の水女子大學自然科學報告"}]}]},"item_11_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_11_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"紀要論文","subitem_description_type":"Other"}]},"item_11_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The structures of asparagine-linked oligosaccharides present on plasma vitronectins of human (hVN) and porcine (pVN) are reported. Oligosac-charides were released from vitronectins by N-glycanase digestion before and after desialylation. The reducing ends of the oligosaccharides were tagged with 2-aminopyridine. Pyridylamino (PA)-derivatives of asialooligosaccharides were fractionated and analyzed by two-dimensional sugar mapping method (N. Tomiya et al. Anal. Biochem., 171, 73-90 (1988)) using reversed-phase and amide-adsorption high performance liquid chromatography (HPLC) in combination with exoglycosidase digestion. More than 70% of the total PA-asialooligosaccharides of both vitronectins were biantennary lactosamine structures. Structures of the major oligosaccharides were confirmed by 400MHz ^1H NMR spectroscopy. PA-sialooligosaccharides were fractionated by anion-exchange and reversed-phase HPLC. Ten and nine major PA-sialooligosaccharides were isolated from hVN and pVN, respectively, and after desialylation their asialo-forms were assigned to PA-asialooligosaccharides by the two-dimensional sugar mapping meth\\\nod. Using desialylation with Salmonella sialidase, which has a kinetic preference for sialyl α2→3 linkages, in combination with acid-desialylation, the linkage and location of sialic acids were presumed by comparing the elution position on HPLC with those of standard PA-sialooligosaccharides and standard derivatives obtained by exoglycosidase digestion. The oligosaccharides of hVN and pVN differed in extent of sialylation; 8% mono-, 70% di- and 22% tri-sialylated for hVN; 7% mono-, 89% di- and 4% tri-sialylated for pVN. Sialic acid was linked dominantly by α2→6 linkage, although α2→3 linkage was also present on both vitronectins. The most remarkable difference between hVN and pVN is the amount of fucose linked to the innermost N-acetylglucosamine by α1→6 linkage.","subitem_description_type":"Other"}]},"item_11_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"70491","nameIdentifierScheme":"WEKO"}],"names":[{"name":"オガワ, ハルコ"}]},{"nameIdentifiers":[{"nameIdentifier":"70492","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ヨネダ, アツコ"}]},{"nameIdentifiers":[{"nameIdentifier":"70493","nameIdentifierScheme":"WEKO"}],"names":[{"name":"マツモト, イサム"}]},{"nameIdentifiers":[{"nameIdentifier":"70494","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ハヤシ, マサオ"}]},{"nameIdentifiers":[{"nameIdentifier":"70495","nameIdentifierScheme":"WEKO"}],"names":[{"name":"イシヅカ, イネオ"}]},{"nameIdentifiers":[{"nameIdentifier":"70496","nameIdentifierScheme":"WEKO"}],"names":[{"name":"セノ, ノブコ"}]}]},"item_11_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"お茶の水女子大学"}]},"item_11_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00033958","subitem_source_identifier_type":"NCID"}]},"item_11_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00298190","subitem_source_identifier_type":"ISSN"}]},"item_11_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"400","subitem_subject_scheme":"NDC"}]},"item_11_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"1367717 bytes"}]},"item_11_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"紀要論文"}]},"item_11_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper"}]},"item_11_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_11_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"03"}]},"item_11_text_79":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_text_value":"Department of Chemistry, Ochanomizu University"},{"subitem_text_value":"Department of Chemistry, Ochanomizu University"},{"subitem_text_value":"Department of Chemistry, Ochanomizu University"},{"subitem_text_value":"Department of Biology, Ochanomizu University"},{"subitem_text_value":"Department of Biochemistry, Teikyo University, School of Medicine"},{"subitem_text_value":"Department of Chemistry, Ochanomizu University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ogawa, Haruko K."}],"nameIdentifiers":[{"nameIdentifier":"70485","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoneda, Atsuko"}],"nameIdentifiers":[{"nameIdentifier":"70486","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumoto, Isamu"}],"nameIdentifiers":[{"nameIdentifier":"70487","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hayashi, Masao"}],"nameIdentifiers":[{"nameIdentifier":"70488","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishizuka, Ineo"}],"nameIdentifiers":[{"nameIdentifier":"70489","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Seno, Nobuko"}],"nameIdentifiers":[{"nameIdentifier":"70490","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-19"}],"displaytype":"detail","filename":"KJ00004470787.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004470787.pdf","url":"https://teapot.lib.ocha.ac.jp/record/34728/files/KJ00004470787.pdf"},"version_id":"ecedd503-2e82-4509-abc6-355c086f5cb9"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The Asparagine-linked Oligosaccharides on Human and Porcine Plasma Vitronectins","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Asparagine-linked Oligosaccharides on Human and Porcine Plasma Vitronectins"}]},"item_type_id":"11","owner":"1","path":["657"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-04-23"},"publish_date":"2007-04-23","publish_status":"0","recid":"34728","relation_version_is_last":true,"title":["The Asparagine-linked Oligosaccharides on Human and Porcine Plasma Vitronectins"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-12T05:39:14.261054+00:00"}