@article{oai:teapot.lib.ocha.ac.jp:00034737, author = {Shimada, Yukiko and Murakami-Murofushi, Kimiko and Shioda, Masaki and Ohta, Jiro}, issue = {2}, journal = {お茶の水女子大學自然科學報告}, month = {Dec}, note = {application/pdf, 紀要論文, Plasmodia of a true slime mold, Physarum polycephalum, synthesize slime polysaccharide and secrete it outside the organisms. This substance was shown to be synthesized and polymerized with cell growth. And when the cells reached a plateau of growth in a nutrient medium, degradation of the polysaccharide and autolysis of the cells occurred. The composition of polysaccharides from different strains of Physarum, F/J or Lu strains, were almost the same, but polymerization rates of them were different. We purified the polysaccharides from these two strains and characterized them. Both of them mainly consisted of galactose (Gal) residue having phosphate (-PO_4) and sulfate (-SO_4) moieties, and the molar ratios of these constituents were 9:1:1 for F/J and 9:2:1 for Lu, respectively. The biological effect of slime polysacchapide was examined and we found that it had a specific inhibitory activity on eukaryotic DNA polymerase α, and degree of inhibition was not dependent on the length of the polysaccharide.}, pages = {39--47}, title = {Extracellular Polysaccharide from a True Slime Mold, Physarum Polycephalum. Polymerization and Auto-Degradation Associated with Cell Growth, and Its Inhibitory Activity on Eukaryotic DNA Polymerase α}, volume = {42}, year = {1991} }