{"created":"2021-03-01T06:40:03.639349+00:00","id":34739,"links":{},"metadata":{"_buckets":{"deposit":"7d9b922d-f205-4013-b1f4-fb0c287bda09"},"_deposit":{"id":"34739","owners":[],"pid":{"revision_id":0,"type":"depid","value":"34739"},"status":"published"},"_oai":{"id":"oai:teapot.lib.ocha.ac.jp:00034739","sets":["347:359:654"]},"item_11_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991-07-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"11","bibliographicPageStart":"1","bibliographicVolumeNumber":"42","bibliographic_titles":[{"bibliographic_title":"お茶の水女子大學自然科學報告"}]}]},"item_11_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_11_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"紀要論文","subitem_description_type":"Other"}]},"item_11_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Sialic acid binding proteins were purified from bovine kidney by successive affinity chromatography on fetuin and heparin clumns. The proteins adsorbed calcium-dependently on fetuin-Sepharose were further subjected to affinity chromatography on heparin-Sepharose. The proteins were separated into two fractions, fraction I and fraction II, by elution with 2mM EDTA and 0.3M NaCl, respectively. The affinity purified fractions had the binding activities to biotinylated fetuin in a polystyrene microtiter well, but not hemagglutinating activities. Inhibition assay of the binding revealed that N-acetylneuraminic acid is the most potent inhibitor for both fractions among the monosaccharides tested. Upon SDS-polyacrylamide gel electrophoresis, fraction-I gave three bands corresponding to 37kDa, 43kDa and 50kDa proteins and fraction-II four bands corresponding to 38kDa, 39kDa, 41kDa and 74kDa proteins. The proteins were electroblotted onto a polyvinylidene difluoride membrane and then subjected to the direct chemical analyses and the binding studies using horseradish peroxidase (HRP)-labeled binding probes. All the proteins had\\\n similar amino acid compositions and were N-terminally blocked. Neither of all proteins was stained with HRP-concanavalin A and HRP-peanut agglutinin, suggesting that they are not glycosylated. All of them were stained with HRP-fetuin and HRP-anhydrotrypsin. The results suggest that they have the carbohydrate recognition domain specific to N-acetylneuraminic acid and are the fragments produced by proteolytic digestion with endogenous trypsin family proteases in the kidney.","subitem_description_type":"Other"}]},"item_11_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"70440","nameIdentifierScheme":"WEKO"}],"names":[{"name":"コジマ, キョウコ"}]},{"nameIdentifiers":[{"nameIdentifier":"70441","nameIdentifierScheme":"WEKO"}],"names":[{"name":"アダチ, マサミ"}]},{"nameIdentifiers":[{"nameIdentifier":"70442","nameIdentifierScheme":"WEKO"}],"names":[{"name":"オガワ, ハルコ"}]},{"nameIdentifiers":[{"nameIdentifier":"70443","nameIdentifierScheme":"WEKO"}],"names":[{"name":"セノ, ノブコ"}]},{"nameIdentifiers":[{"nameIdentifier":"70444","nameIdentifierScheme":"WEKO"}],"names":[{"name":"マツモト, イサム"}]}]},"item_11_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"お茶の水女子大学"}]},"item_11_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00033958","subitem_source_identifier_type":"NCID"}]},"item_11_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00298190","subitem_source_identifier_type":"ISSN"}]},"item_11_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"400","subitem_subject_scheme":"NDC"}]},"item_11_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"933629 bytes"}]},"item_11_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"紀要論文"}]},"item_11_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper"}]},"item_11_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_11_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"03"}]},"item_11_text_79":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_text_value":"From the Department of Chemistry, Faculty of Science, Ochanomizu University"},{"subitem_text_value":"From the Department of Chemistry, Faculty of Science, Ochanomizu University"},{"subitem_text_value":"From the Department of Chemistry, Faculty of Science, Ochanomizu University"},{"subitem_text_value":"From the Department of Chemistry, Faculty of Science, Ochanomizu University"},{"subitem_text_value":"From the Department of Chemistry, Faculty of Science, Ochanomizu University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kojima, Kyoko"}],"nameIdentifiers":[{"nameIdentifier":"70435","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Adachi, Masami"}],"nameIdentifiers":[{"nameIdentifier":"70436","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ogawa, Haruko"}],"nameIdentifiers":[{"nameIdentifier":"70437","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Seno, Nobuko"}],"nameIdentifiers":[{"nameIdentifier":"70438","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumoto, Isamu"}],"nameIdentifiers":[{"nameIdentifier":"70439","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-19"}],"displaytype":"detail","filename":"KJ00004470757.pdf","filesize":[{"value":"933.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004470757.pdf","url":"https://teapot.lib.ocha.ac.jp/record/34739/files/KJ00004470757.pdf"},"version_id":"e70526c9-49f9-46a9-903a-8018292bac57"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Affinity Chromatographic Purification and Characterization of Sialic Acid Binding Proteins in Bovine Kidney","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Affinity Chromatographic Purification and Characterization of Sialic Acid Binding Proteins in Bovine Kidney"}]},"item_type_id":"11","owner":"1","path":["654"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-04-23"},"publish_date":"2007-04-23","publish_status":"0","recid":"34739","relation_version_is_last":true,"title":["Affinity Chromatographic Purification and Characterization of Sialic Acid Binding Proteins in Bovine Kidney"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-12T05:39:14.135749+00:00"}