@article{oai:teapot.lib.ocha.ac.jp:00034899, author = {Akiyama, Fumiko and Seno, Nobuko}, issue = {2}, journal = {お茶の水女子大學自然科學報告}, month = {Dec}, note = {application/pdf, 紀要論文, The mucopolysaccharides from the skins of hagfishes were isolated after pronase digestion and fractionation on a Dowex 1 column. The main mucopolysaccharides were found to be hyaluronic acid and dermatan polysulfate, from the results of electrophoresis, chemical analyses, digestions with hyaluronidase, chondroitinase-AC, -ABC and infrared spectroscopy. This dermatan polysulfate was homogeneous on electrophoresis and gel filtration. The molar ratio of galactosamine : uronic acid : sulfate was about. 1:1:2. On cellulose acetate electrophoresis in 0.2M calcium acetate, this dermatan polysulfate gave a single spot between SChS-B and ChS-H. Its infrared spectrum showed absorption bands for both axial and equatorial sulfate groups, and an absorption band at 800cm^<-1> differing from those of SChS-B and ChS-H. This mucopolysaccharide was not attacked by chondroitinase-AC, whereas it was degraded by chondroitinase-ABC and yielded a new spot "X", which moved more slowly than △Di-diS_D, in addition to spots corresponding to △Di-diS_B, △Di-4S, △Di-diS_E and △Di-6S. The material "X" was characterized as a trisulfated disacchar\ ide : 2-acetamido-2-deoxy-3-O-(2 or 3-O-sulfo-β-D-gluco-4-enepyranosyluronic acid)-4, 6-di-O-sulfo-D-galactose, on the basis of chemical analysis and chondrosulfatase digestion. This mucopolysaccharide is a new type of dermatan polysulfate containing the trisulfated disaccharide unit : 2-acetamido-2-deoxy-3-O-(2 or 3-O-sufo-α-L-idopyranosyluronic acid)-4, 6-di-O-sulfo-D-galactose, two types of disulfated disaccharide units and two types of monosulfated disaccharide units.}, pages = {79--100}, title = {Studies on the Mucopolysaccharides from Hagfish Skin}, volume = {23}, year = {1972} }