@article{oai:teapot.lib.ocha.ac.jp:00034927,
 author = {Ohtawa, Mie and 大多和, 美恵},
 issue = {1},
 journal = {お茶の水女子大學自然科學報告},
 month = {Jul},
 note = {application/pdf, 紀要論文, The metabolism of the myxamoebae, the gamete of the Myxomycete Physarum polycephalum, was investigated by using a Warburg manonometer. The following results were obtained. 1. The Qo_2 value of the myxamoebae on a dry weight basis was about 1.00-1.10 in a medium of 1/30M phosphate buffer at pH 6.0. The Qo_2 value of encysted myxamoebae was very low. 2. The average R.Q. value at pH 5.0 with 1/30M citrate phosphate buffer was found to be 0.99. 3. The optimum pH for the respiration of these myxamoebae was pH 6.0. This rate was almost constant at pH's between 4.0 and 8.0. 4. The respiratory rate of these myxamoebae was enhanced by the addition of extracellular substrates such as glucose, pyruvic acid, succinic acid, malic acid, lactic acid, acetic acid, serine and glutamic acid. The O_2 uptake was strongly inhibited by some metabolic poisons, such as cyanide, natrium azide, monoiodoacetate and 2, 4-dinitrophenol, and was slightly inhibited by sodium fluoride and malonic acid. The presence of cytochromes was confirmed spectrophotoscopically. From these results, the main metabolic pathway of the myxamoebae seems to be as\
 follows: Glucose is decomposed to pyruvic acid through a series of glycolytic enzymes, then pyruvic acid goes into the TCA cycle and is oxidatively decomposed to carbon dioxide with the aid of a cytochrome system. 5. Anaerobic CO_2 production was slight and anaerobic acid production was not observed at all. 6. The metabolism of the myxamoebae is found to be similar to that of the plasmodium, except that they fail to produce acid anaerobically.},
 pages = {59--68},
 title = {Experiments on the Metabolism of the Myxomycete Amoeba},
 volume = {22},
 year = {1971}
}