{"created":"2021-03-01T06:40:31.022628+00:00","id":35136,"links":{},"metadata":{"_buckets":{"deposit":"645408f5-a499-4a49-a888-f9d6cdcebb14"},"_deposit":{"id":"35136","owners":[],"pid":{"revision_id":0,"type":"depid","value":"35136"},"status":"published"},"_oai":{"id":"oai:teapot.lib.ocha.ac.jp:00035136","sets":["347:359:580"]},"item_11_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1952-07","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"92","bibliographicPageStart":"79","bibliographicVolumeNumber":"3","bibliographic_titles":[{"bibliographic_title":"お茶の水女子大學自然科學報告"}]}]},"item_11_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_11_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"紀要論文","subitem_description_type":"Other"}]},"item_11_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Among numerous Streptomyces-strains isolated from soils, F 1001, so-called in our laboratory, exhibited the widest inhibition zone against pathogenic bacteria in the course of our screening-tests. The fact suggests that this strain has an ability to produce the antibiotic in a large amount. Spirals are formed at the ends of hyphae only on the agarmedia extremely poor in nutrients. The hyphae excrete brown pigments that diffuse into the media, when a protein component such as meat extract or peptone is added. From this the strain seems to be of Chromogenus type. Judging from several taxonomic characteristics, we have identified it as one strain of Streptomyces aureus (Waksman and Curtius) group. Cultivation of the strain on 6 kinds of media of different compositions showed the glucose bouillon medium, rendered semicolloidal by adding 0.25% agar, as most suitable for the production of antibiotics. Optimum pH of the broth for growth as well as for potency is 7.0, and optimum temperature for growth is 24℃, while that for potency is 30℃. Broth filtrates inhibit the growth of Staph. aureus in about 20,000,000 dilution, E\\\nscher. coli in about 200,000 dilution. Next we tried to extract active substances out of broth by the usual method and have got a white powder with pale yellowish brown tincture. As a solvent for elution of active substances from charcoal, methanol acidified by hydrochloric acid (pH 2) is excellent, and methanol acidified by sulfuric acid (same pH) falls behind. The powder gives brown colored Sakaguchi-reaction, and forms crystalline reineckate as well as helianthate. It is able to inhibit the growth of all the three groups of pathogenic bacteria i.e., Gram positive, Gram negative and acid-fast bacteria (Mycobacterium tuberculosis bovine type). Lytic action upon bacterial bodies is not so conspicuous. Concerning heat stability, it is indifferent to 3-minute heating at 100℃, while the potency decreases considerably by 5-minute heating at 100℃.","subitem_description_type":"Other"}]},"item_11_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"68911","nameIdentifierScheme":"WEKO"}],"names":[{"name":"オオツキ, トラオ"}]},{"nameIdentifiers":[{"nameIdentifier":"68912","nameIdentifierScheme":"WEKO"}],"names":[{"name":"イマイ, モリエコ"}]}]},"item_11_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"お茶の水女子大学"}]},"item_11_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00033958","subitem_source_identifier_type":"NCID"}]},"item_11_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00298190","subitem_source_identifier_type":"ISSN"}]},"item_11_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"400","subitem_subject_scheme":"NDC"}]},"item_11_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"920670 bytes"}]},"item_11_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"紀要論文"}]},"item_11_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper"}]},"item_11_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_11_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"03"}]},"item_11_text_79":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_text_value":"Botanical Laboratory, Faculty of Science Ochanomizu University"},{"subitem_text_value":"Botanical Laboratory, Faculty of Science Ochanomizu University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大槻, 虎男"}],"nameIdentifiers":[{"nameIdentifier":"68909","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井, 百里江子"}],"nameIdentifiers":[{"nameIdentifier":"68910","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohtsuki, Torao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"68913","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Morieko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"68914","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-19"}],"displaytype":"detail","filename":"KJ00004829307.pdf","filesize":[{"value":"920.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004829307.pdf","url":"https://teapot.lib.ocha.ac.jp/record/35136/files/KJ00004829307.pdf"},"version_id":"cac2b87b-986e-4499-8e0d-7ac2d8746f05"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"抗生物質産生放線状菌の研究 第2報 : F1001號株の培養及びその抗生物質に就いて","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"抗生物質産生放線状菌の研究 第2報 : F1001號株の培養及びその抗生物質に就いて"},{"subitem_title":"Studies on the Antibiotic Producing Actinomycetes II : On the Identification, Culture Condition and Antibiotic Substance of F 1001 Strain","subitem_title_language":"en"}]},"item_type_id":"11","owner":"1","path":["580"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-04-30"},"publish_date":"2008-04-30","publish_status":"0","recid":"35136","relation_version_is_last":true,"title":["抗生物質産生放線状菌の研究 第2報 : F1001號株の培養及びその抗生物質に就いて"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-12T05:40:13.448874+00:00"}