{"created":"2021-03-01T06:49:44.080384+00:00","id":43219,"links":{},"metadata":{"_buckets":{"deposit":"0da0b721-a22e-4b7c-9df7-a7c9f485b430"},"_deposit":{"id":"43219","owners":[],"pid":{"revision_id":0,"type":"depid","value":"43219"},"status":"published"},"_oai":{"id":"oai:teapot.lib.ocha.ac.jp:00043219","sets":["347:359:2400"]},"author_link":["114785","114786"],"control_number":"43219","item_11_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"特別号","bibliographicPageEnd":"20","bibliographicPageStart":"15","bibliographicVolumeNumber":"71","bibliographic_titles":[{"bibliographic_title":"お茶の水女子大学自然科学報告"}]}]},"item_11_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"紀要論文","subitem_description_type":"Other"}]},"item_11_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Coagulation factor XII (FXII) is an 80-kDa plasma glycoprotein, which initiates the intrinsic pathway of blood coagulation reactions. FXII is autocleaved after the Arg353 residue upon contact with a negatively c arged surface, to yield active protease FXII (FXIIa). In this study, we prepared PA-tagged recombinant human FXII (PA-hFXII) in a mammalian expression system using Expi293F cells, analyzed the contact-activation response, protease activity, and glycosylation, and compared them to those of human plasma FXII (hFXII). PA-hFXII was purified using affinity chromatography on anti-PA antibodyconjugated Sepharose from the conditioned medium of the transfected Expi293F cells. PA-hFXII was autoactivated when it came in contact with the negatively charged lipid, sulfatide, and its protease activity was found to be similar to that of hFXII. Two potential N-glycosylation sites underwent glycosylation in both, PA-hFXII and hFXII. Oglycosylation in the proline-rich region appeared more homologous in PA-hFXII than in hFXII. These results showed that recombinant PA-hFXII was suitable for the investigation of the molecular mechanism underlying FXII activation.","subitem_description_type":"Abstract"}]},"item_11_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"お茶の水女子大学"}]},"item_11_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_11_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00033958","subitem_source_identifier_type":"NCID"}]},"item_11_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00298190","subitem_source_identifier_type":"ISSN"}]},"item_11_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"400","subitem_subject_scheme":"NDC"}]},"item_11_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"紀要論文"}]},"item_11_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper"}]},"item_11_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_11_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"03"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NAKAMURA, Sumika","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"114785","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KOJIMA-AIKAWA, Kyoko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"114786","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-10-28"}],"displaytype":"detail","filename":"06,p15-20.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"06,p15-20.pdf","url":"https://teapot.lib.ocha.ac.jp/record/43219/files/06,p15-20.pdf"},"version_id":"6d0ffaec-252f-4880-a6b8-1cf201f691d6"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of Recombinant Human Coagulation Factor XII","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of Recombinant Human Coagulation Factor XII","subitem_title_language":"en"}]},"item_type_id":"11","owner":"1","path":["2400"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-10-28"},"publish_date":"2020-10-28","publish_status":"0","recid":"43219","relation_version_is_last":true,"title":["Characterization of Recombinant Human Coagulation Factor XII"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-02-27T02:22:45.097154+00:00"}