{"created":"2021-03-01T06:06:38.527914+00:00","id":4823,"links":{},"metadata":{"_buckets":{"deposit":"33ab8ea4-daaf-495e-a810-598b35b482ea"},"_deposit":{"id":"4823","owners":[],"pid":{"revision_id":0,"type":"depid","value":"4823"},"status":"published"},"_oai":{"id":"oai:teapot.lib.ocha.ac.jp:00004823","sets":["226:216:221"]},"item_12_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"13","bibliographicPageEnd":"8538","bibliographicPageStart":"8528","bibliographicVolumeNumber":"281","bibliographic_titles":[{"bibliographic_title":"Journal of Biological Chemistry"}]}]},"item_12_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"text/plain","subitem_description_type":"Other"},{"subitem_description":"application/pdf","subitem_description_type":"Other"},{"subitem_description":"application/pdf","subitem_description_type":"Other"},{"subitem_description":"text/plain","subitem_description_type":"Other"}]},"item_12_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"学術雑誌論文","subitem_description_type":"Other"}]},"item_12_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"How glycosylation affects the reactivity of proteins to trypsin is not well understood. Bovine and porcine pancreatic trypsins were discovered to bind to α-Man, Neu5Acα2,6Galβ1,4Glc, and α-Gal sequences by binding studies with biotinylated sugar-polymers. Quantitative kinetic studies supported that phenylmethylsulfonyl fluoride (PMSF)-treated trypsin binds to glycolipid analogues possessing α-Man or α-NeuAc but not to those possessing β-Gal or β-GlcNAc residue. ELISA showed that trypsin binds to six kinds of biotinylated glycoproteins possessing high mannose-type and complex type N-glycans but not to bovine submaxillary mucin, which possesses only O-glycans. Further, the binding of trypsin to glycoproteins was differentially changed by treatments with sequential exoglycosidases, endoglycosidase H, or N-glycosidase F. Quantitative kinetic studies indicated that PMSF-treated trypsin binds with bovine thyroglobulin with the affinity constant of 1010 M-1, which was the highest among the glycoproteins examined, and that α-galactosidase treatment decreased it to 105 M-1. PMSF-treated trypsin bound to other glycoproteins inc\\<br/>luding ovomucoid, a trypsin inhibitor, with the affinity constants of 108-105 mol-1 and were markedly changed by glycosidase treatments in manners consistent with the sugar-binding specificities suggested by ELISA. Thus, the binding site for glycans was shown to be distinct from the catalytic site, allowing trypsin to function as an uncompetitive activator in the hydrolysis of a synthetic peptide substrate. Correspondingly the carbohydrate-binding activities of trypsin were unaffected by treatment with PMSF or soybean trypsin inhibitor. The results indicate the presence of an allosteric regulatory site on trypsin that sugar-specifically interacts with glycoproteins in addition to the proteolytic catalytic site.","subitem_description_type":"Abstract"}]},"item_12_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The American Society for Biochemistry and Molecular Biology"}]},"item_12_relation_54":{"attribute_name":"異版である","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1074/jbc.M513773200","subitem_relation_type_select":"URI"}}]},"item_12_rights_14":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2006 by the American Society for Biochemistry and Molecular Biology."}]},"item_12_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_12_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00251083","subitem_source_identifier_type":"NCID"}]},"item_12_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9258","subitem_source_identifier_type":"ISSN"}]},"item_12_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"464","subitem_subject_scheme":"NDC"}]},"item_12_subject_24":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"生物学","subitem_subject_scheme":"Other"}]},"item_12_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"55515 bytes"},{"subitem_text_value":"27307 bytes"},{"subitem_text_value":"845087 bytes"},{"subitem_text_value":"1836 bytes"}]},"item_12_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"学術雑誌論文"}]},"item_12_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Journal Article"}]},"item_12_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_12_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"01"}]},"item_1622774516477":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takekawa, Hiroko"}],"nameIdentifiers":[{"nameIdentifier":"88927","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ina, Chieko"}],"nameIdentifiers":[{"nameIdentifier":"88928","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sato, Reiko"}],"nameIdentifiers":[{"nameIdentifier":"88929","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Toma, Kazunori"}],"nameIdentifiers":[{"nameIdentifier":"88930","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ogawa, Haruko"}],"nameIdentifiers":[{"nameIdentifier":"88931","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-23"}],"displaytype":"detail","filename":"J. Biol. Chem 281(13).pdf","filesize":[{"value":"845.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"J. Biol. Chem 281(13).pdf","url":"https://teapot.lib.ocha.ac.jp/record/4823/files/J. Biol. 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