@article{oai:teapot.lib.ocha.ac.jp:00004892,
 author = {HAMANO, Takako and TONG, Vincent and MUTAI, Mamoru and HAYASHI, Masao and TANAKA, Eiji},
 issue = {1},
 journal = {Journal of toxicological sciences},
 month = {Feb},
 note = {application/pdf, 学術雑誌論文, To clarify the mechanism of sensitivity to an endotoxin lipopolysaccharide LPS0111 :B4, which causes severe liver injury in a variety of animals, we have developed an in vitro assay to measure Kupffer cell-mediated cytotoxicity in the human liver cell line, WRL68. This assay could detect the decrease in Kupffer cell activity induced by gadolinium chloride (GdCl_3), which is an inhibitor in Kupffer cells. Among Kupffer cells derived from dogs, rats, and monkeys, LPS-activated canine Kupffer cells exhibited remarkably high cytotoxicity against WRL68 cells. This species difference is correlated with a species difference in the lethality of LPS0111 :B4. Additionally, the conditioned medium of LPS-activated canine Kupffer cells was also cytotoxic to WRL68 cells. To identify the mediators of this cytotoxicity, we measured the accelerated release of interleukin- 1β, and interleukin-6 from Kupffer cells on stimulation with LPS0111 :B4. From the correlation of the response to LPS0111 :B4, interleukin-1β and interleukin-6 are considered to be responsible for the canine Kupffer cell-mediated cytotoxicity of LPS0111 :B4.},
 pages = {1--9},
 title = {KUPFFER CELL-MEDIATED CYTOTOXICITY INDUCED BY LIPOPOLYSACCHARIDE0111:B4 IS GREATER IN DOGS THAN IN RATS AND MONKEYS},
 volume = {27},
 year = {2002}
}