{"created":"2021-03-01T06:06:47.825052+00:00","id":4963,"links":{},"metadata":{"_buckets":{"deposit":"5ec32768-14ad-4ff7-9891-0fba79348713"},"_deposit":{"id":"4963","owners":[],"pid":{"revision_id":0,"type":"depid","value":"4963"},"status":"published"},"_oai":{"id":"oai:teapot.lib.ocha.ac.jp:00004963","sets":["226:216:221"]},"item_12_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-02-21","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{"bibliographic_title":"Glycoconjugate Journal"}]}]},"item_12_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"},{"subitem_description":"text/plain","subitem_description_type":"Other"},{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"学術雑誌論文","subitem_description_type":"Other"}]},"item_12_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A proteoglycan (PG) monomer is a macromolecule consisting of one or more glycosaminoglycan (GAG) chains attached to a core protein. PGs have signaling roles and modulatory functions in the extracellular matrix and at the cell surface. To elucidate the functions of higher-order PG structures, pseudoPGs that imitate the PG structure were prepared to develop probes and affinity adsorbents. Poly-L-lysine (PLL) or polyacrylamide (PAA) was coupled with various GAGs, then biotinylated, and the remaining amino groups were blocked to obtain the pseudoPG probes, biotinyl PLL (BPL)- or PAA (BPA)-GAGs. Lactoferrin exhibited 30-times higher affinity toward BPL-heparin than the conventional single-strand probe, biotin-hydrazide-heparin.Heparin-PLL was immobilized on a formyl-Sepharose and compared with the Hep-Sepharose in which heparin was directly immobilized to amino-Sepharose. Screening for ligands in normal rat brain revealed several proteins that specifically bound to either of the two adsorbents, indicating that the heparin-binding proteins exhibit specific recognition depending on the higher-order structure of the PG.","subitem_description_type":"Abstract"}]},"item_12_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"online first / <br/>Received: 28 July 2008 / Revised: 30 September 2008 / Accepted: 8 December 2008 / Published online: 21 February 2009","subitem_description_type":"Other"},{"subitem_description":"The original publication is available at www.springerlink.com","subitem_description_type":"Other"}]},"item_12_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Netherlands"}]},"item_12_relation_54":{"attribute_name":"異版である","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s10719-008-9220-y","subitem_relation_type_select":"URI"}}]},"item_12_relation_63":{"attribute_name":"参照する","attribute_value_mlt":[{"subitem_relation_type":"references","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.springerlink.com/content/880502ul280187v5/","subitem_relation_type_select":"URI"}}]},"item_12_rights_14":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Springer Science + Business Media, LLC 2009"}]},"item_12_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_12_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11981860","subitem_source_identifier_type":"NCID"}]},"item_12_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"02820080","subitem_source_identifier_type":"ISSN"}]},"item_12_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"464","subitem_subject_scheme":"NDC"}]},"item_12_subject_24":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"生物学","subitem_subject_scheme":"Other"}]},"item_12_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"782111 bytes"},{"subitem_text_value":"41649 bytes"},{"subitem_text_value":"27311 bytes"}]},"item_12_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"学術雑誌論文"}]},"item_12_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Journal Article"}]},"item_12_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_12_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"01"}]},"item_1622774516477":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakagawa, Keiko"}],"nameIdentifiers":[{"nameIdentifier":"88918","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakamura, Kosuke"}],"nameIdentifiers":[{"nameIdentifier":"88919","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Haishima, Yuji"}],"nameIdentifiers":[{"nameIdentifier":"88920","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamagami, Makiko"}],"nameIdentifiers":[{"nameIdentifier":"88921","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saito, Kana"}],"nameIdentifiers":[{"nameIdentifier":"88922","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakagami, Hiromi"}],"nameIdentifiers":[{"nameIdentifier":"88923","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ogawa, Haruko"}],"nameIdentifiers":[{"nameIdentifier":"88924","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-23"}],"displaytype":"detail","filename":"Glycoconjugate Journal  2009 Feb 21. [Epub ahead of print].doc.pdf","filesize":[{"value":"782.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Glycoconjugate Journal  2009 Feb 21. [Epub ahead of print].doc.pdf","url":"https://teapot.lib.ocha.ac.jp/record/4963/files/Glycoconjugate Journal  2009 Feb 21. [Epub ahead of print].doc.pdf"},"version_id":"b8008202-1248-4e38-9d60-a93095ce50e5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Glycosaminoglycan","subitem_subject_scheme":"Other"},{"subitem_subject":"Heparin","subitem_subject_scheme":"Other"},{"subitem_subject":"binding proteins","subitem_subject_scheme":"Other"},{"subitem_subject":"Cyclophilin A","subitem_subject_scheme":"Other"},{"subitem_subject":"HSP90","subitem_subject_scheme":"Other"},{"subitem_subject":"Amphoterin","subitem_subject_scheme":"Other"},{"subitem_subject":"Detection and separation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Pseudoproteoglycan (pseudoPG) probes that simulate PG macromolecular structure for screening and isolation of PG-binding proteins","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Pseudoproteoglycan (pseudoPG) probes that simulate PG macromolecular structure for screening and isolation of PG-binding proteins","subitem_title_language":"en"}]},"item_type_id":"12","owner":"1","path":["221"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-01-26"},"publish_date":"2018-01-26","publish_status":"0","recid":"4963","relation_version_is_last":true,"title":["Pseudoproteoglycan (pseudoPG) probes that simulate PG macromolecular structure for screening and isolation of PG-binding proteins"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-12T06:03:23.656500+00:00"}