WEKO3
アイテム
Matrix glycoprotein differentially changes in liver regeneration and cirrhosis : Glycosylation alters ligand binding in matrix remodeling
http://hdl.handle.net/10083/2362
http://hdl.handle.net/10083/2362bc399796-b156-4c02-b5d7-526c6286a203
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004830730.pdf (337.5 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2008-04-30 | |||||
| タイトル | ||||||
| タイトル | Matrix glycoprotein differentially changes in liver regeneration and cirrhosis : Glycosylation alters ligand binding in matrix remodeling | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Suzuki, Risa
× Suzuki, Risa× Yamada, Sadako× Uchibori-Iwaki, Haruhi× Oda-Tamai, Sachie× Kato, Shigemi× Akamatsu, Nobu× Ogawa, Haruko |
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| 作成者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70769 | |||||
| 姓名 | スズキ, リサ | |||||
| 作成者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70770 | |||||
| 姓名 | ヤマダ, サダコ | |||||
| 作成者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70771 | |||||
| 姓名 | ウチボリ(イワキ), ハルヒ | |||||
| 作成者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70772 | |||||
| 姓名 | オダ(タマイ), サチエ | |||||
| 作成者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70773 | |||||
| 姓名 | カトウ, シゲミ | |||||
| 作成者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70774 | |||||
| 姓名 | アカマツ, ノブ | |||||
| 作成者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70775 | |||||
| 姓名 | オガワ, ハルコ | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Background Vitronectins (VN) are multifunctional adhesive glycoproteins that are present in plasma and the extracellular matrix of most tissues. We previously reported that the collagen-binding activity of VN is enhanced by a change in glycosylation in vitro and during liver regeneration after partial hepatectomy in vivo [Uchibori-Iwaki et al., Glycobiology (2000) 10, 865-874]. Plasma concentrations of VN declined in rats during liver regeneration 24 h after partial hepatectomy, while carbohydrate concentrations of VN decreased to 2/3 of that in sham-operated rats. Carbohydrate composition and lectin reactivity indicated that the N-glycan structures and sialylation significantly changed without affecting the peptide portion after partial hepatectomy. VN from partially hepatectomized rats were found to exhibit markedly enhanced binding to type I collagen. The enzymatic deglycosylation of VN demonstrated that collagen binding increased by 1.2 times after deN-glycosylation of VN, while it increased by more than 2.9 times after desialylation. To elucidate the biological significance of glycan modulation, changes of VN\ in cirrhosis were studied and compared with those during liver regeneration. Methods VNs purified from patients' and normal plasma were examined for plasma concentration and collagen-binding activity by ELISA, reactivity against lectins by dot blotting, and carbohydrate composition. Results Plasma concentrations of VN declined in chronic liver diseases in the order of hepatitis>cirrhosis>hepatocellular carcinoma with cirrhosis. Lectin reactivity and carbohydrate analyses of purified VN. from cirrhotic plasma (LC-VN) indicated that sialylation was elevated. LC-VN exhibited decreased binding to type I collagen. Collagen-binding studies of plasma before and after urea-treatment indicated that the active form of VN increased in cirrhotic plasma. Conclusions The attenuated collagen-binding activity of LC-VN is attributable to a change of glycosylation. The increase of active VN in cirrhotic plasma may contribute to the matrix incorporation of VN. These findings suggest that the collagen binding activity of VN is modulated by the alteration of peptide glycosylation during liver regeneration after partial hepatectomy and during the pathological processes, which may contribute to the tissue remodeling processes. |
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| 書誌情報 |
お茶の水女子大學自然科學報告 巻 53, 号 1, p. 63-66, 発行日 2002-06 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00298190 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00033958 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 値 | 337502 bytes | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 400 | |||||
| 出版者 | ||||||
| 出版者 | お茶の水女子大学 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 紀要論文 | |||||
| 資源タイプ・ローカル | ||||||
| 値 | 紀要論文 | |||||
| 資源タイプ・NII | ||||||
| 値 | Departmental Bulletin Paper | |||||
| 資源タイプ・DCMI | ||||||
| 値 | text | |||||
| 資源タイプ・ローカル表示コード | ||||||
| 値 | 03 | |||||
| 所属 | ||||||
| 値 | Graduate School of Humanities and Sciences, Graduate School of Ochanomizu University | |||||
| 所属 | ||||||
| 値 | Department of Pathological Science and Technology, Faculty of Medicine, Tottori University | |||||
| 所属 | ||||||
| 値 | Graduate School of Humanities and Sciences, Graduate School of Ochanomizu University | |||||
| 所属 | ||||||
| 値 | Department of Biochemistry, St. Marianna University School of Medicine | |||||
| 所属 | ||||||
| 値 | Department of Biochemistry, St. Marianna University School of Medicine | |||||
| 所属 | ||||||
| 値 | Department of Biochemistry, St. Marianna University School of Medicine | |||||
| 所属 | ||||||
| 値 | Graduate School of Humanities and Sciences, Graduate School of Ochanomizu University | |||||
