WEKO3
アイテム
Studies on Chicken Liver Lectin
http://hdl.handle.net/10083/2322
http://hdl.handle.net/10083/2322d95bfcf9-023e-4628-9342-cd057cc21073
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004830608.pdf (1.1 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-04-30 | |||||
| タイトル | ||||||
| タイトル | Studies on Chicken Liver Lectin | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 著者 |
Kitagaki, Haruko
× Kitagaki, Haruko× Matsumoto, Isamu× Sasaki, Hitomi× Seno, Nobuko |
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| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70318 | |||||
| 姓名 | キタガキ, ハルコ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70319 | |||||
| 姓名 | マツモト, イサム | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70320 | |||||
| 姓名 | ササキ, ヒトミ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70321 | |||||
| 姓名 | セノオ, ノブコ | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | A lectin highly reactive with dermatan sulfate (DS-lectin) was purified from adult chicken liver using new affinity adsorbents which were prepared by immobilizing dermatan sulfate or heparin at the reducing ends on hydrazino-Toyopearl. The DS-lectin behaved as a single protein with a molecular weight of approx. 180,000 by gel chromatography, but could be further dissociated into three proteins with apparent molecular weights of 40,000, 47,000 and 87,000 on SDS polyacrylamide gel electrophoresis. On excitation at 280nm, the DS-lectin emitted fluorescence centered at 336nm, which was attributable to tryptophan residues and could be quenched by the addition of specific saccharides. The association constants of the DS-lectin with specific saccharides were calculated from changes in intensities of fluorescence-difference spectra induced by the saccharides. Dermatan sulfate and protuberic acid, which is composed of L-iduronic acid and D-glucuronic acid (1:2), had the highest association constants among polysaccharides tested. Partially N-desulfated heparin had a higher association constant than that of native heparin whi\ le dextran sulfate had no affinity. D-Glucuronic acid and N-acetylneuraminic acid induced weak but significant quenching, but not N-acetylgalactosamine or cellobiose. These results were essentially in agreement with those of hemagglutination inhibition tests and indicated that DS-lectin has a strong affinity for L-iduronic acid residues and probably carboxyl groups in the saccharides, while sulfate groups on the saccharides interfere with the specific interaction. |
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| 書誌情報 |
お茶の水女子大學自然科學報告 巻 36, 号 1, p. 89-102, 発行日 1985-07 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00298190 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00033958 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 1122585 bytes | ||||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 400 | |||||
| 出版者 | ||||||
| 出版者 | お茶の水女子大学 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 紀要論文 | |||||
| 資源タイプ・ローカル | ||||||
| 紀要論文 | ||||||
| 資源タイプ・NII | ||||||
| Departmental Bulletin Paper | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 03 | ||||||
| 所属 | ||||||
| Department of Chemistry, Faculty of Science, Ochanomizu University | ||||||
| 所属 | ||||||
| Department of Chemistry, Faculty of Science, Ochanomizu University | ||||||
| 所属 | ||||||
| Department of Chemistry, Faculty of Science, Ochanomizu University | ||||||
| 所属 | ||||||
| Department of Chemistry, Faculty of Science, Ochanomizu University | ||||||
