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  1. 紀要
  2. お茶の水女子大學自然科學報告
  3. 20(2)

Studies on the Composition and Structure of Whale Cartilage Keratosulfate

http://hdl.handle.net/10083/2155
http://hdl.handle.net/10083/2155
8485c3b9-5323-45c5-af0b-9b3d76b29c4c
名前 / ファイル ライセンス アクション
KJ00004829690.pdf KJ00004829690.pdf (1.9 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2008-04-30
タイトル
タイトル Studies on the Composition and Structure of Whale Cartilage Keratosulfate
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 Toda, Noriko

× Toda, Noriko

WEKO 69810

Toda, Noriko

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戸田, 紀子

× 戸田, 紀子

WEKO 69812

en 戸田, 紀子

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著者(ヨミ)
識別子Scheme WEKO
識別子 69811
姓名 トダ, ノリコ
内容記述
内容記述タイプ Other
内容記述 From whale nasal cartilage, keratosulfate barium salt was obtained in good yield by ethanol fractionation on cellulose column. The keratosulfate was further fractionated by anion exchange (Dowex 1) column chromatography. All the fractions obtained from the Dowex 1 column contained the various amounts of galactosamine, sialic acid, mannose, fucose and protein, in addition to the constituents ; glucosamine, galactose and sulfate. The ratios of sulfate to hexosamine in each fraction differed from 0.75 to 1.8. As sugar sulfates, galactose 6-sulfate other than N-acetylglucosamine 6-sulfate was detected in partial acid hydrolyzate by electrophoresis and by paper chromatography. The high content (11.7%) of sialic acid was remarkable in contrast with corneal keratosulfate in which the content of sialic acid was less than 1%. The sialic acid was isolated from whale cartilage keratosulfate and identified to be N-acetylneuraminic acid by paper chromatography, color reactions and infrared spectrum. The results of pronase and alkali treatments suggest that the sialic acid may be present in the carbohydrate moiety and not in the\
alkali labile peptide moiety of the keratosulfate. The sialic acid was easily released by neuraminidase digestion, which indicates that the sialic acid attaches to the non-reducing end of carbohydrate. In connection with sialic acid in cartilage, the sialic acid contents of cartilages from various sources were determined. The linkage between carbohydrate and peptide in the keratosulfate fraction was investigated by the action of alkali in the presence and absence of sodium borohydride. N-acetylhexosamine seemed to link to threonine and serine, and N-acetylhexosamine was assumed to be N-acetylgalactosamine.
書誌情報 お茶の水女子大學自然科學報告

巻 20, 号 2, p. 69-98, 発行日 1969-12
ISSN
収録物識別子タイプ ISSN
収録物識別子 00298190
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00033958
フォーマット
内容記述タイプ Other
内容記述 application/pdf
形態
1863795 bytes
日本十進分類法
主題Scheme NDC
主題 400
出版者
出版者 お茶の水女子大学
資源タイプ
内容記述タイプ Other
内容記述 紀要論文
資源タイプ・ローカル
紀要論文
資源タイプ・NII
Departmental Bulletin Paper
資源タイプ・DCMI
text
資源タイプ・ローカル表示コード
03
所属
Department of Chemistry, Faculty of Science, Ochanomizu University
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