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Studies on the Composition and Structure of Whale Cartilage Keratosulfate
http://hdl.handle.net/10083/2155
http://hdl.handle.net/10083/21558485c3b9-5323-45c5-af0b-9b3d76b29c4c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004829690.pdf (1.9 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-04-30 | |||||
| タイトル | ||||||
| タイトル | Studies on the Composition and Structure of Whale Cartilage Keratosulfate | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 著者 |
Toda, Noriko
× Toda, Noriko× 戸田, 紀子 |
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| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 69811 | |||||
| 姓名 | トダ, ノリコ | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | From whale nasal cartilage, keratosulfate barium salt was obtained in good yield by ethanol fractionation on cellulose column. The keratosulfate was further fractionated by anion exchange (Dowex 1) column chromatography. All the fractions obtained from the Dowex 1 column contained the various amounts of galactosamine, sialic acid, mannose, fucose and protein, in addition to the constituents ; glucosamine, galactose and sulfate. The ratios of sulfate to hexosamine in each fraction differed from 0.75 to 1.8. As sugar sulfates, galactose 6-sulfate other than N-acetylglucosamine 6-sulfate was detected in partial acid hydrolyzate by electrophoresis and by paper chromatography. The high content (11.7%) of sialic acid was remarkable in contrast with corneal keratosulfate in which the content of sialic acid was less than 1%. The sialic acid was isolated from whale cartilage keratosulfate and identified to be N-acetylneuraminic acid by paper chromatography, color reactions and infrared spectrum. The results of pronase and alkali treatments suggest that the sialic acid may be present in the carbohydrate moiety and not in the\ alkali labile peptide moiety of the keratosulfate. The sialic acid was easily released by neuraminidase digestion, which indicates that the sialic acid attaches to the non-reducing end of carbohydrate. In connection with sialic acid in cartilage, the sialic acid contents of cartilages from various sources were determined. The linkage between carbohydrate and peptide in the keratosulfate fraction was investigated by the action of alkali in the presence and absence of sodium borohydride. N-acetylhexosamine seemed to link to threonine and serine, and N-acetylhexosamine was assumed to be N-acetylgalactosamine. |
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| 書誌情報 |
お茶の水女子大學自然科學報告 巻 20, 号 2, p. 69-98, 発行日 1969-12 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00298190 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00033958 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 1863795 bytes | ||||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 400 | |||||
| 出版者 | ||||||
| 出版者 | お茶の水女子大学 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 紀要論文 | |||||
| 資源タイプ・ローカル | ||||||
| 紀要論文 | ||||||
| 資源タイプ・NII | ||||||
| Departmental Bulletin Paper | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 03 | ||||||
| 所属 | ||||||
| Department of Chemistry, Faculty of Science, Ochanomizu University | ||||||
