WEKO3
アイテム
Annexins and Heparan Sulfate Proteoglycans in Pancreatic Endocrine β Cells
http://hdl.handle.net/10083/2389
http://hdl.handle.net/10083/2389cca335e0-da61-49e9-afc7-1ba0f609cf4e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004830782.pdf (1.3 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-04-30 | |||||
| タイトル | ||||||
| タイトル | Annexins and Heparan Sulfate Proteoglycans in Pancreatic Endocrine β Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 著者 |
Shimura, Yumiko Eris
× Shimura, Yumiko Eris× Ida, Michiru× Kimata, Koji× Matsumoto, Isamu |
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| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70872 | |||||
| 姓名 | シムラ, ユミコ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70873 | |||||
| 姓名 | イダ, ミチル | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70874 | |||||
| 姓名 | キマタ, コウジ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70875 | |||||
| 姓名 | マツモト, イサム | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | We previously demonstrated that annexins are localized in exocrine tissues such as kidney, liver, intestine and pancreatic acinar cells. We also elucidated the glycosaminoglycan (GAG) binding properties of annexins IV, V and VI and showed that each of them has its own specific binding activities to GAGS. In this study, we examined the function of annexin VI and GAGs in pancreatic β cells. Immunohistochemical studies and immunoblot assays showed that annexin VI localized in rat pancreatic islets, HIT-T15 cells derived from rat insulinorna and MIN6 cells derived from mouse insulinoma. Immunoblot assay detected heparan sulfate proteoglycan in those cell lysates but not chondroitin sulfate proteoglycan. Affinity adsorption assay showed annexin VI bound to the heparan sulfate proteoglycans derived from HIT-T15 cells indicating that these proteoglycans the endogenous ligand of annexin VI in β cells. Insulin secretion assay from permeabilized MIN6 cells showed that full-length human annexin VI promotes insulin release, but not other mutants. Inhibition of Ca^<2+>-dependent insulin release by GAGs was inferred that GAGs bl\ ocked binding of annexin VI and membrane vesicle. These results suggest that annexin VI was considered to exit in pancreatic endocrine cells, and to interact with both cell-extracellular matrix heparan sulfate proteoglycan and membrane vesicle in β cells. |
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| 書誌情報 |
お茶の水女子大學自然科學報告 巻 55, 号 2, p. 41-58, 発行日 2005-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00298190 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00033958 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 1314615 bytes | ||||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 400 | |||||
| 出版者 | ||||||
| 出版者 | お茶の水女子大学 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 紀要論文 | |||||
| 資源タイプ・ローカル | ||||||
| 紀要論文 | ||||||
| 資源タイプ・NII | ||||||
| Departmental Bulletin Paper | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 03 | ||||||
| 所属 | ||||||
| Graduate School Of Humanities and Sciences | ||||||
| 所属 | ||||||
| Graduate School Of Humanities and Sciences | ||||||
| 所属 | ||||||
| Institute for Molecular Science of Medicine, Aichi Medical University | ||||||
| 所属 | ||||||
| Graduate School Of Humanities and Sciences:Department of Chemistry, Faculty of Science, Ochanomizu University | ||||||
