WEKO3
アイテム
Multi-specificities of the N-Acetylglucosamine-Binding Lectin from Psathyrella velutina Mushroom toward Acidic Glycoconjugates
http://hdl.handle.net/10083/861
http://hdl.handle.net/10083/861c41d9452-ece1-4292-9ab8-48f622f5779b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004470905.pdf (1.2 MB)
|
|
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-04-23 | |||||
| タイトル | ||||||
| タイトル | Multi-specificities of the N-Acetylglucosamine-Binding Lectin from Psathyrella velutina Mushroom toward Acidic Glycoconjugates | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 著者 |
Ueda, Haruko
× Ueda, Haruko× Kojima, Kyoko× Saitoh, Takeshi× Ogawa, Haruko |
|||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70663 | |||||
| 姓名 | ウエダ, ハルコ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70664 | |||||
| 姓名 | コジマ, キョウコ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70665 | |||||
| 姓名 | サイトウ, タケシ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70666 | |||||
| 姓名 | オガワ, ハルコ | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | A lectin from the fruiting body of Psathyrella velutina (PVL) has been reported to be specific for nonreducing terminal N-acetylglucosamine (GlcNAc) residue. PVL was found to exhibit multispecificity toward acidic glycoconjugates, i.e., polysaccharides, sialoglycoproteins, and sulfatide. PVL was purified by single-step affinity chromatography on an N-acetylchitooligosaccharides (GlcNAc_<5-6>)-Sepharose column. The binding specificities of PVL were studied using neoproteoglycans and neoglycoproteins involving heparin or GlcNAc_<5-6>. PVL was shown, by both membrane analysis and solid phase assay, to bind with heparin neoproteoglycans. The binding was inhibited by polygalacturonic acid, dextran sulfate, or fucoidan as well as heparin, but not by GlcNAc, other glycosaminoglycans, colominic acid, or DNA. The pH-dependencies of the binding to heparin and to GlcNAc_<5-6> were different. Biotinylation of PVL destroyed the heparin binding activity while retaining the GlcNAc binding activity. In addition, circular dichroism spectroscopy of PVL indicated that a small conformational change of PVL is induced by binding with GlcN\ Ac but not with heparin, suggesting that the binding sites for acidic polysaccharides and GlcNAc are independently located on PVL. On the other hand. PVL bound to sialoglycoproteins on solid phase assays, and the binding was inhibited with GlcNAc or desialylation treatment, indicating that PVL recognizes N-acetylneuraminic acid residue in glycoproteins at GlcNAc-binding site. The binding of PVL to sulfatide was not inhibited by GlcNAc, N-acetylneuraminic acid, or heparin. These findings indicate that the binding specificities of PVL to glycoconjugates are quite different from those reported for oligosaccharides and PVL is a novel member of muluspecific lectin in higher fungi. |
|||||
| 書誌情報 |
お茶の水女子大學自然科學報告 巻 50, 号 1, p. 31-45, 発行日 1999-07-15 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00298190 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00033958 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 1178164 bytes | ||||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 400 | |||||
| 出版者 | ||||||
| 出版者 | お茶の水女子大学 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 紀要論文 | |||||
| 資源タイプ・ローカル | ||||||
| 紀要論文 | ||||||
| 資源タイプ・NII | ||||||
| Departmental Bulletin Paper | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 03 | ||||||
| 所属 | ||||||
| Department of Advanced Biosciences, Graduate School of Humanities and Sciences, Ochanomizu University | ||||||
| 所属 | ||||||
| Department of Chemistry, Faculty of Science, Ochanomizu University | ||||||
| 所属 | ||||||
| The Mushroom Research Institute of Japan | ||||||
| 所属 | ||||||
| Department of Advanced Biosciences, Graduate School of Humanities and Sciences, Ochanomizu University | ||||||
