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STIMULATION OF Gq PROTEIN-COUPLED SIGNAL TRANSDUCTION BY CYCLIC PHOSPHATIDIC ACID (cPA) AND ACTIVATION OF PROTEIN KINASE C IN VITRO
http://hdl.handle.net/10083/850
http://hdl.handle.net/10083/8507df55c37-240f-4ce8-af82-8713e19ad365
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004470877.pdf (652.2 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-04-23 | |||||
| タイトル | ||||||
| タイトル | STIMULATION OF Gq PROTEIN-COUPLED SIGNAL TRANSDUCTION BY CYCLIC PHOSPHATIDIC ACID (cPA) AND ACTIVATION OF PROTEIN KINASE C IN VITRO | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cyclic phophatidic acid (cPA) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PHYLPA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | protein kinase C (PKC) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Gq protein | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | signal transduction | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | LPA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | lysophosphatidic acid, cPA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cyclic lysophosphatidic acid, DG | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | diacyl-glycerol, PKC | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | protein kinase C, cPKC | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | conventional PKC, nPKC | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | novel PKC, PS | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | phophatidylserine, TPA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | tetradecanoylphorbol 13-acetate, bFGF | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | basic fibroblast growth factor | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 著者 |
Murakami-Murofushi, Kimiko
× Murakami-Murofushi, Kimiko× Uchiyama, Naoko× Kobayashi, Tetsuyuki× Murofushi, Hiromu |
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| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70613 | |||||
| 姓名 | ムラカミ-ムロフシ, キミコ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70614 | |||||
| 姓名 | ウチヤマ, ナオコ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70615 | |||||
| 姓名 | コバヤシ, テツユキ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70616 | |||||
| 姓名 | ムロフシ, ヒロム | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | A unique cyclic phosphatidic acid (cPA), named PHYLPA, which was originally isolated from myxoamoebae of a true slime mold, Physarum polycephalum, and composed of cyclic phosphate and cyclopropane-containing hexadecanoic acid (1-3), was shown to stimulate the Gq protein-coupled signal transduction system and activity of protein kinase C (PKC) in vitro. When fibroblast cells were treated with PHYLPA, phosphoinositide hydrolysis and Ca^<2+> mobilization were immediately occurred, and a specific PKC substrate, MARKS, was markedly phosphorylated. Some derivatives of PHYLPA synthesized chemically also showed the obvious stimulatory effects on MARKS phosphorylation. These results show that cPA may act on a cell surface, specific receptor which activates Gq protein, a member of trimeric G protein, and phosphoinositide breakdown and PKC activation occur subsequently. While, cPA obviously stimulated purified PKC activity, and the extent of activation was much higher than that by usual lysophosphatidic acid (LPA) or diacylglycerol (DG). This indicates the possibility that the cPA may be generated by the lipolytic action from p\ lasma membrane and activates PKC directly in the cell. cPA activated both conventional PKC (cPKC) and novel PKC (nPKC), former of which has Ca^<2+> -regulatory domain, and latter one has not, at different extents. One of the stereoisomers of natural PHYLPA, assigned as sodium 3-O-[(9'S,10'R)-9',10'-methanohexadecanoyl]-sn-glycerol 1,2-cyclic phosphate, showed an apparently high stimulatory activity on these enzymes. |
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| 書誌情報 |
お茶の水女子大學自然科學報告 巻 48, 号 2, p. 29-38, 発行日 1998-03-12 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00298190 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00033958 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 652248 bytes | ||||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 400 | |||||
| 出版者 | ||||||
| 出版者 | お茶の水女子大学 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 紀要論文 | |||||
| 資源タイプ・ローカル | ||||||
| 紀要論文 | ||||||
| 資源タイプ・NII | ||||||
| Departmental Bulletin Paper | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 03 | ||||||
| 所属 | ||||||
| Department of Biology, Faculty of Science, Ochanomizu University | ||||||
| 所属 | ||||||
| Department of Biology, Faculty of Science, Ochanomizu University | ||||||
| 所属 | ||||||
| Department of Biology, Faculty of Science, Ochanomizu University | ||||||
| 所属 | ||||||
| Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo | ||||||
