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Purification and Characterization of an Acid Protease from Myxoamoebae of a True Slime Mold, Physarum polycephalum
http://hdl.handle.net/10083/837
http://hdl.handle.net/10083/8374f4f334f-6841-4b5b-b10b-b9fa986d2460
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004470823.pdf (747.4 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-04-23 | |||||
| タイトル | ||||||
| タイトル | Purification and Characterization of an Acid Protease from Myxoamoebae of a True Slime Mold, Physarum polycephalum | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 著者 |
Minowa-Sonobe, Yukie
× Minowa-Sonobe, Yukie× Shimada, Ynkiko× Murakami-Murofushi, Kimiko |
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| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70556 | |||||
| 姓名 | ミノワ-ソノベ, ユキエ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70557 | |||||
| 姓名 | シマダ, ユキコ | |||||
| 著者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70558 | |||||
| 姓名 | ムラカミ-ムロフシ, キミコ | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | In order to compare the characteristics of acid protease (s) in diploid and haploid cells of a true slime mold, Physarum polycephalum, an acid protease was purified from the haploid myxoamoebae of Physarum by a combination of detergent extraction, acid precipitation, and ion-exchange column chromatographies. The purified enzyme having a molecular weight of 68,000 was composed of two polypeptide chains, 31-kDa heavy chain and 23-kDa light chain. These polypeptides were cross-linked by disulfide bond (s), and the heavy chain contained carbohydrate moiety composed of mannose, glucosamine, fucose and glucose. Optimum pH of the enzyme reaction was 1.7 toward hemoglobin as a substrate. A typical aspartic protease inhibitor, diazoacetyl-D, L-norleucine methyl ester (DAN), inhibited the enzyme activity in the presence of cupric ions, but the enzyme was not sensitive to the other aspartic protease inhibitors, 1, 2-epoxy-3-(p-nitrophenoxy) propane (EPNP) and pepstatin A. These results indicate that the characteristics of the amoeboid protease is very similar to those of the plasmodial enzyme. In addition, amino acid compositio\ n and specific activity of the purified enzyme were also very similar to those of the plasmodial protease. |
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| 書誌情報 |
お茶の水女子大學自然科學報告 巻 45, 号 1, p. 67-78, 発行日 1994-07-15 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00298190 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00033958 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 747381 bytes | ||||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 400 | |||||
| 出版者 | ||||||
| 出版者 | お茶の水女子大学 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 紀要論文 | |||||
| 資源タイプ・ローカル | ||||||
| 紀要論文 | ||||||
| 資源タイプ・NII | ||||||
| Departmental Bulletin Paper | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 03 | ||||||
| 所属 | ||||||
| Department of Biology, Faculty of Science, Ochanomizu University | ||||||
| 所属 | ||||||
| Department of Biology, Faculty of Science, Ochanomizu University | ||||||
| 所属 | ||||||
| Department of Biology, Faculty of Science, Ochanomizu University | ||||||
