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Studies on Solanum tuberosum Agglutinin
http://hdl.handle.net/10083/2301
http://hdl.handle.net/10083/23013bfc1a53-fa11-4d7f-b9c0-2b96242681cb
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004830512.pdf (1.2 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-04-30 | |||||
| タイトル | ||||||
| タイトル | Studies on Solanum tuberosum Agglutinin | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Matsumoto, Isamu
× Matsumoto, Isamu× Jimbo, Akiko× Mizuno, Yuko× Seno, Nobuko× Jeanloz, Roger W. |
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| 作成者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70258 | |||||
| 姓名 | マツモト, イサム | |||||
| 作成者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70259 | |||||
| 姓名 | ジンボ, アキコ | |||||
| 作成者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70260 | |||||
| 姓名 | ミズノ, ユウコ | |||||
| 作成者(ヨミ) | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 70261 | |||||
| 姓名 | セノオ, ノブコ | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Potato lectin (Solanum tuberosum agglutinin, STA) was purified by affinity chromatography on (GlcNAc)_3-Sepharose 6B. The molecular weight of STA was estimated to be about 100,000 by gel filtration on Sephadex G-150 in 0.05M phosphate buffered saline, pH 7.2. By sedimentation equilibrium analysis, it was shown that STA was an aggregating system with a monomer molecular weight of 54,000. Equilibrium dialysis showed that STA (dimer) has two binding sites for a specific sugar per molecule. STA had a high content of sugar, most of which was arabinose, and was rich in Hyp and Cys. Upon interaction with specific sugars, STA induced a UV-difference spectrum having positive peaks at 292nm and 285nm, which seemed to be characteristic of the tryptophyl residue. The association constants of STA with chitin oligosaccharides were determined from the intensities of the difference spectra at various concentrations of sugars. They increased with increasing chain-length of the sugar. Frontal affinity chromatography of STA was performed on columns of immobilized chitin oligosaccharides and that of chitin oligosaccharides on a column\ of immobilized STA. The association constants obtained with STA-Sepharose were in good agreement with those obtained by difference spectra, whereas those obtained with (GlcNAc)_2, 3-Sepharose were much higher, presumably owing to the effect of multivalency of ligands. The analyses of CD spectra of STA in the far UV region indicated the presence of approximately 40% of β and 60% of unordered form, and no evidence of α-helix conformation. This confirms the structure suggested by the unusual amino acid composition and by the high content of sugar. |
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| 書誌情報 |
お茶の水女子大學自然科學報告 巻 33, 号 1/2, p. 55-75, 発行日 1982-12 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00298190 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00033958 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 値 | 1223696 bytes | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 400 | |||||
| 出版者 | ||||||
| 出版者 | お茶の水女子大学 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 紀要論文 | |||||
| 資源タイプ・ローカル | ||||||
| 値 | 紀要論文 | |||||
| 資源タイプ・NII | ||||||
| 値 | Departmental Bulletin Paper | |||||
| 資源タイプ・DCMI | ||||||
| 値 | text | |||||
| 資源タイプ・ローカル表示コード | ||||||
| 値 | 03 | |||||
| 所属 | ||||||
| 値 | Department of Chemistry, Faculty of Science, Ochanomizu University | |||||
| 所属 | ||||||
| 値 | Department of Chemistry, Faculty of Science, Ochanomizu University | |||||
| 所属 | ||||||
| 値 | Department of Chemistry, Faculty of Science, Ochanomizu University | |||||
| 所属 | ||||||
| 値 | Department of Chemistry, Faculty of Science, Ochanomizu University | |||||
| 所属 | ||||||
| 値 | Laboratory for Carbohydrate Research, Departments of Biological Chemistry and Medicine, Harvard Medical School and Massachusetts General Hospital | |||||
