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Characterization of Recombinant Human Coagulation Factor XII
http://hdl.handle.net/10083/00064013
http://hdl.handle.net/10083/000640134480d3b7-4eb8-49b8-ae06-12da317793c3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
06,p15-20.pdf (2.0 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-10-28 | |||||
| タイトル | ||||||
| タイトル | Characterization of Recombinant Human Coagulation Factor XII | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
NAKAMURA, Sumika
× NAKAMURA, Sumika× KOJIMA-AIKAWA, Kyoko |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Coagulation factor XII (FXII) is an 80-kDa plasma glycoprotein, which initiates the intrinsic pathway of blood coagulation reactions. FXII is autocleaved after the Arg353 residue upon contact with a negatively c arged surface, to yield active protease FXII (FXIIa). In this study, we prepared PA-tagged recombinant human FXII (PA-hFXII) in a mammalian expression system using Expi293F cells, analyzed the contact-activation response, protease activity, and glycosylation, and compared them to those of human plasma FXII (hFXII). PA-hFXII was purified using affinity chromatography on anti-PA antibodyconjugated Sepharose from the conditioned medium of the transfected Expi293F cells. PA-hFXII was autoactivated when it came in contact with the negatively charged lipid, sulfatide, and its protease activity was found to be similar to that of hFXII. Two potential N-glycosylation sites underwent glycosylation in both, PA-hFXII and hFXII. Oglycosylation in the proline-rich region appeared more homologous in PA-hFXII than in hFXII. These results showed that recombinant PA-hFXII was suitable for the investigation of the molecular mechanism underlying FXII activation. | |||||
| 書誌情報 |
お茶の水女子大学自然科学報告 巻 71, 号 特別号, p. 15-20, 発行日 2020-09 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00298190 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00033958 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 400 | |||||
| 出版者 | ||||||
| 出版者 | お茶の水女子大学 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 紀要論文 | |||||
| 資源タイプ・ローカル | ||||||
| 値 | 紀要論文 | |||||
| 資源タイプ・NII | ||||||
| 値 | Departmental Bulletin Paper | |||||
| 資源タイプ・DCMI | ||||||
| 値 | text | |||||
| 資源タイプ・ローカル表示コード | ||||||
| 値 | 03 | |||||
