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Pseudoproteoglycan (pseudoPG) probes that simulate PG macromolecular structure for screening and isolation of PG-binding proteins
http://hdl.handle.net/10083/35135
http://hdl.handle.net/10083/35135b2395de2-9a0e-492a-befc-0b55f850667a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Glycoconjugate Journal 2009 Feb 21. [Epub ahead of print].doc.pdf (782.1 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2018-01-26 | |||||
| タイトル | ||||||
| タイトル | Pseudoproteoglycan (pseudoPG) probes that simulate PG macromolecular structure for screening and isolation of PG-binding proteins | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Glycosaminoglycan | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Heparin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | binding proteins | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Cyclophilin A | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | HSP90 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Amphoterin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Detection and separation | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
Nakagawa, Keiko
× Nakagawa, Keiko× Nakamura, Kosuke× Haishima, Yuji× Yamagami, Makiko× Saito, Kana× Sakagami, Hiromi× Ogawa, Haruko |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A proteoglycan (PG) monomer is a macromolecule consisting of one or more glycosaminoglycan (GAG) chains attached to a core protein. PGs have signaling roles and modulatory functions in the extracellular matrix and at the cell surface. To elucidate the functions of higher-order PG structures, pseudoPGs that imitate the PG structure were prepared to develop probes and affinity adsorbents. Poly-L-lysine (PLL) or polyacrylamide (PAA) was coupled with various GAGs, then biotinylated, and the remaining amino groups were blocked to obtain the pseudoPG probes, biotinyl PLL (BPL)- or PAA (BPA)-GAGs. Lactoferrin exhibited 30-times higher affinity toward BPL-heparin than the conventional single-strand probe, biotin-hydrazide-heparin.Heparin-PLL was immobilized on a formyl-Sepharose and compared with the Hep-Sepharose in which heparin was directly immobilized to amino-Sepharose. Screening for ligands in normal rat brain revealed several proteins that specifically bound to either of the two adsorbents, indicating that the heparin-binding proteins exhibit specific recognition depending on the higher-order structure of the PG. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | online first / <br/>Received: 28 July 2008 / Revised: 30 September 2008 / Accepted: 8 December 2008 / Published online: 21 February 2009 | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The original publication is available at www.springerlink.com | |||||
| 書誌情報 |
Glycoconjugate Journal 発行日 2009-02-21 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 02820080 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11981860 | |||||
| 権利 | ||||||
| 権利情報 | Springer Science + Business Media, LLC 2009 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | text/plain | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 782111 bytes | ||||||
| 形態 | ||||||
| 41649 bytes | ||||||
| 形態 | ||||||
| 27311 bytes | ||||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 著者版フラグ | ||||||
| 値 | author | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 464 | |||||
| NIIサブジェクト | ||||||
| 主題Scheme | Other | |||||
| 主題 | 生物学 | |||||
| 出版者 | ||||||
| 出版者 | Springer Netherlands | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 学術雑誌論文 | |||||
| 資源タイプ・ローカル | ||||||
| 学術雑誌論文 | ||||||
| 資源タイプ・NII | ||||||
| Journal Article | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 01 | ||||||
| 異版である | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://doi.org/10.1007/s10719-008-9220-y | |||||
| 参照する | ||||||
| 関連タイプ | references | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.springerlink.com/content/880502ul280187v5/ | |||||
